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Literature summary for 2.7.7.12 extracted from
- Molecular cloning and biochemical characterization of galactose-1-phosphate uridylyltransferase from Gracilaria changii (Rhodophyta) (2012), J. Phycol., 48, 155-162.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene GALT, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli strain BL21(DE3) plysS | Gracilaria changii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics forward and reverse reaction, overview | Gracilaria changii | |
| 0.051 | - |
UDP-alpha-D-galactose | pH 7.5, 25°C | Gracilaria changii |  |
| 0.092 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 25°C | Gracilaria changii | |
| 0.116 | - |
alpha-D-galactose 1-phosphate | pH 7.5, 25°C | Gracilaria changii | |
| 0.134 | - |
UDP-alpha-D-glucose | pH 7.5, 25°C | Gracilaria changii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required, enzyme-bound | Gracilaria changii | |
| Mg2+ | required | Gracilaria changii | |
| Zn2+ | required, enzyme-bound | Gracilaria changii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate | Gracilaria changii | - |
alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gracilaria changii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) plysS | Gracilaria changii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | - |
Gracilaria changii | UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate | - |
r | |
| UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate | - |
Gracilaria changii | alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | homology modeling of the three-dimensional protein structure, overview | Gracilaria changii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| galactose-1-phosphate uridylyltransferase | - |
Gracilaria changii |
| GALT | - |
Gracilaria changii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Gracilaria changii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Gracilaria changii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | homology modeling of the three-dimensional protein structure, overview | Gracilaria changii |
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Release 2023.1 (January 2023)
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