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Literature summary for 2.7.7.12 extracted from

  • Satomura, T.; Hiraki, A.; Kawai, T.; Kawakami, R.; Ohshima, T.; Sakuraba, H.
    Expression, purification, crystallization and preliminary X-ray diffraction analysis of a galactose 1-phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum (2012), Acta Crystallogr. Sect. F, 68, 330-332.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.12

Cloned(Commentary)

Cloned (Comment) Organism
gene PAE1184, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21 (DE3) Codon Plus RIL Pyrobaculum aerophilum

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, sitting-drop vapour-diffusion method, 10mg/ml protein, 4°C, X-ray diffraction structure determination and analysis at 2.73 A resolution Pyrobaculum aerophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Pyrobaculum aerophilum
59000
-
 gel filtration Pyrobaculum aerophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate Pyrobaculum aerophilum
-
 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 r
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate Pyrobaculum aerophilum DSM 7523
-
 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 r

Organism

Organism UniProt Comment Textmining
Pyrobaculum aerophilum Q8ZXN7
-
-
Pyrobaculum aerophilum DSM 7523 Q8ZXN7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21 (DE3) Codon Plus RIL by heat treatment at 80°C for 10 min, followed by anion exchange and cation exchange chromatography Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate
-
 Pyrobaculum aerophilum alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 r
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate
-
 Pyrobaculum aerophilum DSM 7523 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 36000, SDS-PAGE Pyrobaculum aerophilum

Synonyms

Synonyms Comment Organism
galactose-1-phosphate uridylyltransferase
-
 Pyrobaculum aerophilum
GALT
-
 Pyrobaculum aerophilum

General Information

General Information Comment Organism
metabolism the enzyme catalyzes a pivotal step in the Leloir pathway of galactose metabolism, overview Pyrobaculum aerophilum