Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants as His-tagged proteins in Escherichia coli Bl21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | - |
Homo sapiens |
additional information | introduction of Asn and Arg at residue 188, normally Gln, in a three-dimensional model of the Escherichia coli enzyme-UMP-crystal | Escherichia coli |
Q188N | site-directed mutagenesis, reduced activity | Homo sapiens |
Q188R | most common native mutation causing galactosemia in the white population | Homo sapiens |
Q188R | site-directed mutagenesis for expression of the mutant in Escherichia coli, nearly no activity in vitro | Homo sapiens |
Q188R | native mutant, no enzyme activity in vivo, galactosemia phenotype | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens | |
additional information | - |
additional information | wild-type and mutants in the 2-step reaction | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09148 | Protein Data Base: 1HXQ, three-dimensional model of the Escherichia coli enzyme-UMP-crystal | - |
Homo sapiens | - |
native galactosemic Q188R mutant | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type, partial 67fold, and mutants from Escherichia coli Bl21(DE3) | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | mechanism, modeling | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0006 | - |
partially purified recombinant mutant Q188R | Homo sapiens |
0.03 | - |
partially purified recombinant mutant Q188N | Homo sapiens |
0.08 | - |
partially purified recombinant wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + alpha-D-galactose 1-phosphate | 2-step mechanism | Homo sapiens | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GALT | - |
Homo sapiens |