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Literature summary for 2.7.7.12 extracted from
- Purification and properties of hexose 1-phosphate uridylyltransferase from Bifidobacterium bifidum (1978), Agric. Biol. Chem., 42, 723-730.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| glycerol 20% stabilizes | Bifidobacterium bifidum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | 94% inhibition at 0.2 mM | Bifidobacterium bifidum |  |
| iodoacetate | no inhibition | Bifidobacterium bifidum | |
| additional information | no inhibition by NaF | Bifidobacterium bifidum | |
| NEM | no inhibition | Bifidobacterium bifidum | |
| PCMB | 42% inhibition at 0.2 mM | Bifidobacterium bifidum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
UDP-glucose | pH 8.7, 25°C | Bifidobacterium bifidum | |
| 0.031 | - |
UDP-galactose | pH 8.7, 25°C | Bifidobacterium bifidum | |
| 0.14 | 0.16 | alpha-D-glucose 1-phosphate | pH 8.7, 25°C | Bifidobacterium bifidum | |
| 0.3 | 0.5 | alpha-D-galactose 1-phosphate | pH 8.7, 25°C | Bifidobacterium bifidum | |
| 6.66 | - |
alpha-D-galactose 1-phosphate | pH 8.7, 25°C | Bifidobacterium bifidum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
gel filtration | Bifidobacterium bifidum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + alpha-D-galactose 1-phosphate | Bifidobacterium bifidum | involved in conversion of galactose into alpha-D-glucose 1-phosphate, Leloir pathway | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium bifidum | - |
constitutive expression | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 213fold to homogeneity | Bifidobacterium bifidum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.53 | - |
purified enzyme | Bifidobacterium bifidum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 0.01 M phosphate buffer, pH 7.5: 40% loss of activity within 2 weeks and 60% within a month, with 20% glycerol: 15% loss of activity within 2 months | Bifidobacterium bifidum |
| 4°C, in 0.01 M potassium phosphate buffer, pH 7.5, inactivation within 2 weeks, 2-mercaptoethanol, DTT and 20% glycerol slightly stabilize | Bifidobacterium bifidum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-glucose + alpha-D-galactose 1-phosphate | no activity | Bifidobacterium bifidum | alpha-D-glucose 1-phosphate + CDP-galactose | - |
? | |
| additional information | no activity with CDP-glucose | Bifidobacterium bifidum | ? | - |
? | |
| additional information | no activity with ADP-glucose or GDP-glucose | Bifidobacterium bifidum | ? | - |
? | |
| TDP-glucose + alpha-D-galactose 1-phosphate | reaction at 60% the rate of UDP-glucose | Bifidobacterium bifidum | alpha-D-glucose 1-phosphate + TDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | highly specific for the substrates | Bifidobacterium bifidum | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | involved in conversion of galactose into alpha-D-glucose 1-phosphate, Leloir pathway | Bifidobacterium bifidum | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 53000-55000, SDS-PAGE | Bifidobacterium bifidum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bifidobacterium bifidum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
DTT does not prevent heat inactivation | Bifidobacterium bifidum |
| 40 | - |
10 min, 20% loss of activity | Bifidobacterium bifidum |
| 50 | - |
above, inactivation | Bifidobacterium bifidum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bifidobacterium bifidum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | 9 | about half-maximal activity at pH 4.4 and pH 9.0 | Bifidobacterium bifidum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
and below, very unstable | Bifidobacterium bifidum |
| 6 | 9 | stable | Bifidobacterium bifidum |
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