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Literature summary for 2.7.7.107 extracted from

  • Rice, K.; Batul, K.; Whiteside, J.; Kelso, J.; Papinski, M.; Schmidt, E.; Pratasouskaya, A.; Wang, D.; Sullivan, R.; Bartlett, C.; Weadge, J.; Van der Kamp, M.; Moreno-Hagelsieb, G.; Suits, M.; Horsman, G.
    The predominance of nucleotidyl activation in bacterial phosphonate biosynthesis (2019), Nat. Commun., 10, 3698 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.107

Crystallization (Commentary)

Crystallization (Comment) Organism
PntC structural analyses reveal steric discrimination against phosphocholine, substrate of EC 2.7.7.15 Lancefieldella rimae
structures determined in apo form and in the presence of CTP and (2-aminoethyl)phosphonate reveal steric discrimination against phosphocholine, substrate of EC 2.7.7.15. Water, residues Glu196 and Glu104 are positioned to interact with the primary amine of (2-aminoethyl)phosphonate via hydrogen bonds Treponema denticola

Protein Variants

Protein Variants Comment Organism
K153A less than 10% of wild-type activity Treponema denticola
K25A less than 10% of wild-type activity Treponema denticola
R15A less than 10% of wild-type activity Treponema denticola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 (2-aminoethyl)phosphonate pH 7.8, temperature not specified in the publication Lancefieldella rimae
0.016
-
 (2-aminoethyl)phosphonate pH 7.8, temperature not specified in the publication Treponema denticola

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ presence of two adjacent magnesium ions in the PntC active site Treponema denticola

Organism

Organism UniProt Comment Textmining
Lancefieldella rimae
-
-
-
Lancefieldella rimae ATCC 49626
-
-
-
Treponema denticola
-
-
-
Treponema denticola ATCC 35405
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + (2-aminoethyl)phosphonate
-
 Treponema denticola diphosphate + CMP-(2-aminoethyl)phosphonate
-
 ?
CTP + (2-aminoethyl)phosphonate
-
 Lancefieldella rimae diphosphate + CMP-(2-aminoethyl)phosphonate
-
 ?
CTP + (2-aminoethyl)phosphonate
-
 Lancefieldella rimae ATCC 49626 diphosphate + CMP-(2-aminoethyl)phosphonate
-
 ?
CTP + (2-aminoethyl)phosphonate
-
 Treponema denticola ATCC 35405 diphosphate + CMP-(2-aminoethyl)phosphonate
-
 ?
additional information enzyme displays more than 400fold specificity for (2-aminoethyl)phosphonate over choline phosphate, substrate of EC 2.7.7.15 Lancefieldella rimae ?
-
-
additional information enzyme displays more than 400fold specificity for (2-aminoethyl)phosphonate over choline phosphate, substrate of EC 2.7.7.15 Lancefieldella rimae ATCC 49626 ?
-
-

Synonyms

Synonyms Comment Organism
pntC
-
 Treponema denticola
pntC
-
 Lancefieldella rimae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.05
-
 (2-aminoethyl)phosphonate pH 7.8, temperature not specified in the publication Treponema denticola
3.7
-
 (2-aminoethyl)phosphonate pH 7.8, temperature not specified in the publication Lancefieldella rimae

General Information

General Information Comment Organism
physiological function enzyme activates (2-aminoethyl)phosphonate for incorporation into cell wall phosphonoglycans and phosphonolipids Treponema denticola
physiological function enzyme activates (2-aminoethyl)phosphonate for incorporation into cell wall phosphonoglycans and phosphonolipids Lancefieldella rimae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
68
-
 (2-aminoethyl)phosphonate pH 7.8, temperature not specified in the publication Treponema denticola
320
-
 (2-aminoethyl)phosphonate pH 7.8, temperature not specified in the publication Lancefieldella rimae