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Literature summary for 2.7.7.103 extracted from
- Manganese-induced substrate promiscuity in the reaction catalyzed by phosphoglutamine cytidylyltransferase from Campylobacter jejuni (2019), Biochemistry, 58, 2144-2151 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Cj1416, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Campylobacter jejuni subsp. jejuni |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | limited activity | Campylobacter jejuni subsp. jejuni |  |
| Mn2+ | required, can replace Mg2+, manganese-induced promiscuity of Cj1416 | Campylobacter jejuni subsp. jejuni | |
| additional information | limited activity with Mg2+, expansion in the substrate profile of Cj1416 when the reaction is catalyzed in the presence of MnCTP | Campylobacter jejuni subsp. jejuni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + N5-phospho-L-glutamine | Campylobacter jejuni subsp. jejuni | - |
diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine | - |
? | |
| CTP + N5-phospho-L-glutamine | Campylobacter jejuni subsp. jejuni NCTC 11168 | - |
diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine | - |
? | |
| CTP + N5-phospho-L-glutamine | Campylobacter jejuni subsp. jejuni ATCC 700819 | - |
diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Campylobacter jejuni subsp. jejuni | Q0P8J8 | serotype O2 | - |
| Campylobacter jejuni subsp. jejuni ATCC 700819 | Q0P8J8 | serotype O2 | - |
| Campylobacter jejuni subsp. jejuni NCTC 11168 | Q0P8J8 | serotype O2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Campylobacter jejuni subsp. jejuni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 3'-phosphocytidine 5'-diphosphoramidate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + 3'-phospho-5'-cytidine diphosphoramidate | - |
? | |
| CTP + 3'-phosphocytidine 5'-diphosphoramidate | - |
Campylobacter jejuni subsp. jejuni NCTC 11168 | diphosphate + 3'-phospho-5'-cytidine diphosphoramidate | - |
? | |
| CTP + 3'-phosphocytidine 5'-diphosphoramidate | - |
Campylobacter jejuni subsp. jejuni ATCC 700819 | diphosphate + 3'-phospho-5'-cytidine diphosphoramidate | - |
? | |
| CTP + 3-phospho-D-glycerate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + 2-hydroxy-2-oxo-1,3,2lambda5-dioxaphospholane-4-carboxylic acid | - |
? | |
| CTP + 3-phospho-D-glycerate | - |
Campylobacter jejuni subsp. jejuni NCTC 11168 | diphosphate + 2-hydroxy-2-oxo-1,3,2lambda5-dioxaphospholane-4-carboxylic acid | - |
? | |
| CTP + 3-phospho-D-glycerate | - |
Campylobacter jejuni subsp. jejuni ATCC 700819 | diphosphate + 2-hydroxy-2-oxo-1,3,2lambda5-dioxaphospholane-4-carboxylic acid | - |
? | |
| CTP + 3-phospho-D-glycerate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + CDP-3-D-glycerate | - |
? | |
| CTP + arsenate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + ? | - |
? | |
| CTP + ethanolamine phosphate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + CDP-ethanolamine | - |
? | |
| CTP + ethanolamine phosphate | - |
Campylobacter jejuni subsp. jejuni NCTC 11168 | diphosphate + CDP-ethanolamine | - |
? | |
| CTP + ethanolamine phosphate | - |
Campylobacter jejuni subsp. jejuni ATCC 700819 | diphosphate + CDP-ethanolamine | - |
? | |
| CTP + glycerol-1-phosphate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + 2-hydroxy-4-(hydroxymethyl)-1,3,2lambda5-dioxaphospholan-2-one + CMP | - |
? | |
| CTP + glycerol-1-phosphate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + CDP-glycerol | - |
? | |
| CTP + glycerol-2-phosphate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + 2-hydroxy-4-(hydroxymethyl)-1,3,2lambda5-dioxaphospholan-2-one + CMP | - |
? | |
| CTP + glycerol-2-phosphate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + CDP-2-glycerol | - |
? | |
| CTP + L-serine phosphate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + CDP-L-serine | - |
? | |
| CTP + methyl phosphate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + O-methyl-CDP | - |
? | |
| CTP + methyl phosphonate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + CMP methyl phosphonate | - |
? | |
| CTP + N5-phospho-L-glutamine | - |
Campylobacter jejuni subsp. jejuni | diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine | - |
? | |
| CTP + N5-phospho-L-glutamine | - |
Campylobacter jejuni subsp. jejuni NCTC 11168 | diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine | - |
? | |
| CTP + N5-phospho-L-glutamine | - |
Campylobacter jejuni subsp. jejuni ATCC 700819 | diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine | - |
? | |
| CTP + phosphate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + CDP | - |
? | |
| CTP + phosphoramidate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + cytidine 5'-diphosphoramidate | - |
? | |
| CTP + serinol phosphate | - |
Campylobacter jejuni subsp. jejuni | diphosphate + CDP-serinol | - |
? | |
| additional information | in the presence of MnCTP, Cj1416 can directly catalyze the formation of cytidine diphosphoramidate from phosphoramidate and MnCTP. In addition, it catalyzes the formation of 12 different reaction products using L-glutamine phosphate, phosphoramidate, methyl phosphate, methyl phosphonate, phosphate, arsenate, ethanolamine phosphate, glycerol-1-phosphate, glycerol-2-phosphate, serinol phosphate, L-serine phosphate, or 3-phospho-D-glycerate as the nucleophile to displace pyrophosphate from CTP. No activity is detected with MgATP as a substrate. Expansion in the substrate profile of Cj1416 when the reaction is catalyzed in the presence of MnCTP, substrate specificity of Cj1416, overview. NMR product analysis | Campylobacter jejuni subsp. jejuni | ? | - |
- |
|
| additional information | in the presence of MnCTP, Cj1416 can directly catalyze the formation of cytidine diphosphoramidate from phosphoramidate and MnCTP. In addition, it catalyzes the formation of 12 different reaction products using L-glutamine phosphate, phosphoramidate, methyl phosphate, methyl phosphonate, phosphate, arsenate, ethanolamine phosphate, glycerol-1-phosphate, glycerol-2-phosphate, serinol phosphate, L-serine phosphate, or 3-phospho-D-glycerate as the nucleophile to displace pyrophosphate from CTP. No activity is detected with MgATP as a substrate. Expansion in the substrate profile of Cj1416 when the reaction is catalyzed in the presence of MnCTP, substrate specificity of Cj1416, overview. NMR product analysis | Campylobacter jejuni subsp. jejuni NCTC 11168 | ? | - |
- |
|
| additional information | in the presence of MnCTP, Cj1416 can directly catalyze the formation of cytidine diphosphoramidate from phosphoramidate and MnCTP. In addition, it catalyzes the formation of 12 different reaction products using L-glutamine phosphate, phosphoramidate, methyl phosphate, methyl phosphonate, phosphate, arsenate, ethanolamine phosphate, glycerol-1-phosphate, glycerol-2-phosphate, serinol phosphate, L-serine phosphate, or 3-phospho-D-glycerate as the nucleophile to displace pyrophosphate from CTP. No activity is detected with MgATP as a substrate. Expansion in the substrate profile of Cj1416 when the reaction is catalyzed in the presence of MnCTP, substrate specificity of Cj1416, overview. NMR product analysis | Campylobacter jejuni subsp. jejuni ATCC 700819 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cj1416 | - |
Campylobacter jejuni subsp. jejuni |
| CTP/phosphoglutamine cytididylyltransferase | - |
Campylobacter jejuni subsp. jejuni |
| phosphoglutamine cytidylyltransferase | - |
Campylobacter jejuni subsp. jejuni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Campylobacter jejuni subsp. jejuni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Campylobacter jejuni subsp. jejuni |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | Campylobacter jejuni, is a Gram-negative pathogen that contains a unique O-methyl phosphoramidate (MeOPN) on its capsular polysaccharide. Enzyme CTP/phosphoglutamine cytididylyltransferase (Cj1416) catalyzes the second step of the biosynthesis pathway, the displacement of diphosphate from MgCTP by L-glutamine phosphate to form CDP-L-glutamine | Campylobacter jejuni subsp. jejuni |
| physiological function | Campylobacter jejuni, is a Gram-negative pathogen that contains a unique O-methyl phosphoramidate (MeOPN) on its capsular polysaccharide. Enzyme CTP/phosphoglutamine cytididylyltransferase (Cj1416) catalyzes the second step of the biosynthesis pathway, the displacement of diphosphate from MgCTP by L-glutamine phosphate to form CDP-L-glutamine | Campylobacter jejuni subsp. jejuni |
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