Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.102 extracted from

  • Boudet, J.; Devillier, J.C.; Wiegand, T.; Salmon, L.; Meier, B.H.; Lipps, G.; Allain, F.H.
    A small helical bundle prepares primer synthesis by binding two nucleotides that enhance sequence-specific recognition of the DNA template (2019), Cell, 176, 154-166.e13 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP ATP binding enhances sequence-specific DNA recognition for primase anchoring Sulfolobus islandicus

Cloned(Commentary)

Cloned (Comment) Organism
-
Sulfolobus islandicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ssDNA + n dNTP Sulfolobus islandicus primases have a fundamental role in DNA replication. They synthesize a primer that is then extended by DNA polymerases ssDNA/pppdN(pdN)n-1 hybrid + (n-1) diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Sulfolobus islandicus Q54324
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus islandicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ssDNA + n dNTP primases have a fundamental role in DNA replication. They synthesize a primer that is then extended by DNA polymerases Sulfolobus islandicus ssDNA/pppdN(pdN)n-1 hybrid + (n-1) diphosphate
-
?
ssDNA + n dNTP a small helical bundle prepares primer synthesis by binding two nucleotides that enhance sequence-specific recognition of the DNA template. Archaeoeukaryotic primases require for synthesis a catalytic and an accessory domain. For the pRN1 archaeal primase, this domain is a 115-amino acid helix bundle domain (HBD). Only the HBD binds the DNA template. DNA binding becomes sequence-specific after a major allosteric change in the HBD, triggered by the binding of two nucleotide triphosphates Sulfolobus islandicus ssDNA/pppdN(pdN)n-1 hybrid + (n-1) diphosphate
-
?

Synonyms

Synonyms Comment Organism
primase HBD
-
Sulfolobus islandicus

General Information

General Information Comment Organism
physiological function primases have a fundamental role in DNA replication. They synthesize a primer that is then extended by DNA polymerases Sulfolobus islandicus