Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfolobus islandicus |
Crystallization (Comment) | Organism |
---|---|
- |
Sulfolobus islandicus |
Protein Variants | Comment | Organism |
---|---|---|
H141A | loss of primase activity | Sulfolobus islandicus |
W314A | poor primase activity | Sulfolobus islandicus |
W314F | reduced primase activity | Sulfolobus islandicus |
W314Y | reduced primase activity | Sulfolobus islandicus |
W347A | no reduction in primase activity | Sulfolobus islandicus |
W361A | no reduction in primase activity | Sulfolobus islandicus |
Y352A | loss of primase activity | Sulfolobus islandicus |
Y352F | no reduction in primase activity | Sulfolobus islandicus |
Y352H | loss of primase activity | Sulfolobus islandicus |
Y352W | loss of primase activity | Sulfolobus islandicus |
Y367A | no reduction in primase activity | Sulfolobus islandicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus islandicus | Q54324 | encoded on plasmid pRN1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-TTTTTTTTGTGCACTTT + n dNTP | - |
Sulfolobus islandicus | 5'-TTTTTTTTGTGCACTTT/pppdN(pdN)n-1 + (n-1) diphosphate | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | The minimal region of the protein required for primase activity encompasses amino-acid residues 40-370. The C-terminal part of the minimal region folds into a compact domain with six helices and is stabilized by a disulfide bond. The C-terminal helix of the helix bundle domain is required for primase activity | Sulfolobus islandicus |