Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | presence of ATP is required | Sulfolobus islandicus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfolobus islandicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
dATP | 50°C, pH 7.5, synthesis of full-length primer | Sulfolobus islandicus | |
0.15 | - |
ATP | 50°C, pH 7.5, synthesis of full-length primer | Sulfolobus islandicus | |
0.2 | - |
5'-CTTCTTCTGTGC-3' | 50°C, pH 7.5, synthesis of full-length primer | Sulfolobus islandicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus islandicus | Q54324 | encoded on plasmid pRN1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-CTTCTTCTGTGC-3' + n dNTP | i.e. minimal substrate which supports synthesis of the full-length primer. The base 3' to the central GTG motif is critical for primer synthesis and the bases 5' of the GTG determine the length of the primer or run-off product | Sulfolobus islandicus | 5'-CTTCTTCTGTGC-3'/pppdN(pdN)n-1 + (n-1) diphosphate | - |
? | |
M13 ssDNA + n ATP | - |
Sulfolobus islandicus | M13 ssDNA/pppN(pA)n-1 + (n-1) diphosphate | - |
? | |
M13 ssDNA + n dATP | - |
Sulfolobus islandicus | M13 ssDNA/pppdN(pdA)n-1 + (n-1) diphosphate | - |
? | |
M13 ssDNA + n dNTP | - |
Sulfolobus islandicus | M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate | - |
? | |
M13 ssDNA + n NTP | - |
Sulfolobus islandicus | M13 ssDNA/pppN(pN)n-1 + (n-1) diphosphate | - |
? | |
additional information | homopolymers as substrates do not yield detectable primase activity | Sulfolobus islandicus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
5'-CTTCTTCTGTGC-3' | 50°C, pH 7.5, synthesis of full-length primer | Sulfolobus islandicus | |
0.11 | - |
ATP | 50°C, pH 7.5, synthesis of full-length primer | Sulfolobus islandicus | |
0.16 | - |
dATP | 50°C, pH 7.5, synthesis of full-length primer | Sulfolobus islandicus |
General Information | Comment | Organism |
---|---|---|
physiological function | the primase activity synthesizes a mixed primer consisting of a single ribonucleotide at the 5' end followed by seven deoxynucleotides. Ribonucleotides and deoxynucleotides are strictly required at the respective positions within the primer. The primase activity is highly sequence-specific and requires the trinucleotide motif GTG in the template. Primer synthesis starts outside of the recognition motif, immediately 5' to the recognition motif. Non-complementary bases are not incorporated into the primer | Sulfolobus islandicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
5'-CTTCTTCTGTGC-3' | 50°C, pH 7.5, synthesis of full-length primer | Sulfolobus islandicus | |
0.016 | - |
ATP | 50°C, pH 7.5, synthesis of full-length primer | Sulfolobus islandicus | |
0.023 | - |
dATP | 50°C, pH 7.5, synthesis of full-length primer | Sulfolobus islandicus |