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Literature summary for 2.7.7.102 extracted from

  • Desogus, G.; Onesti, S.; Brick, P.; Rossi, M.; Pisani, F.
    Identification and characterization of a DNA primase from the hyperthermophilic archaeon Methanococcus jannaschii (1999), Nucleic Acids Res., 27, 4444-4450 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.102

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 5fold and 2fold activation on substrates poly(dT) and poly(dC), respectively, at 20 mM Methanocaldococcus jannaschii
Mn2+ required, optimium concentration 10 mM Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58249 small subunit PriS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
M13 ssDNA + n dNTP
-
 Methanocaldococcus jannaschii M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate
-
 ?
additional information no substrate: poly(A). In the pH range 6.0-7.6, main products are oilgoribonucleotides of 7 and 14 bases. At pH 8.1 and pH 8.5, longer products are also detected Methanocaldococcus jannaschii ?
-
 ?
poly(dC)2500 + n GTP
-
 Methanocaldococcus jannaschii poly(dC)2500/pppG(pG)n-1 + n-1 diphosphate
-
 ?
poly(dT)400 + n ATP
-
 Methanocaldococcus jannaschii poly(dT)400/pppA(pA)n-1 + n-1 diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 44700, recombinant subunit PriS, SDS-PAGE Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 substrate poly(dT) Methanocaldococcus jannaschii
60 75 substrate poly(dC) Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, 20% loss of activity Methanocaldococcus jannaschii
70
-
 1 h, 30% loss of activity Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
-
 Methanocaldococcus jannaschii