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Literature summary for 2.7.7.102 extracted from

  • Lao-Sirieix, S.; Bell, S.
    The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities (2004), J. Mol. Biol., 344, 1251-1263 .
    View publication on PubMed
Search for more literature for 2.7.7.102

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of both subunits in Saccharomyces cerevisiae and Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
D101 catalytic site mutant, complete loss of activity Saccharolobus solfataricus
D103 catalytic site mutant, complete loss of activity Saccharolobus solfataricus
N175 residue involved in DNA-binding. Mutant accumulates dinucleotides, no other products are formed Saccharolobus solfataricus
R176 residue involved in DNA binding. Mutant accumulates dinucleotides, no other products are formed Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97Z83 and Q9UWW1 Q97Z83 i.e. small subunit PriS, Q9UWW1 i.e. large subunit PriL
-
Saccharolobus solfataricus DSM 1617 Q97Z83 and Q9UWW1 Q97Z83 i.e. small subunit PriS, Q9UWW1 i.e. large subunit PriL
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
M13 ssDNA + n dNTP
-
 Saccharolobus solfataricus M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate
-
 ?
M13 ssDNA + n dNTP
-
 Saccharolobus solfataricus DSM 1617 M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate
-
 ?
M13 ssDNA + n NTP
-
 Saccharolobus solfataricus M13 ssDNA/pppN(pN)n-1 + (n-1) diphosphate
-
 ?
M13 ssDNA + n NTP
-
 Saccharolobus solfataricus DSM 1617 M13 ssDNA/pppN(pN)n-1 + (n-1) diphosphate
-
 ?
phage X174 ssDNA + n NTP
-
 Saccharolobus solfataricus phage X174 ssDNA/pppN(pN)n-1 + (n-1) diphosphate
-
 ?
phage X174 ssDNA + n NTP
-
 Saccharolobus solfataricus DSM 1617 phage X174 ssDNA/pppN(pN)n-1 + (n-1) diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 35700, subunit PriL, plus x * 37600, subunit PriS Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function PriSL is capable of utilising both ribonucleotides and deoxyribonucleotides for primer synthesis in the presence of natural, or synthetic, single-stranded DNA. Products range from dinucleotides to DNA molecules in excess of 7 kb and RNA up to 1 kb in length. PriSL has a significantly higher affinity for ribonucleotides than for deoxyribonucleotides Saccharolobus solfataricus