Literature summary for 2.7.7.102 extracted from

Liu, L.; Komori, K.; Ishino, S.; Bocquier, A.; Cann, I.; Kohda, D.; Ishino, Y.
The archaeal DNA primase Biochemical characterization of the p41-p46 complex from Pyrococcus furiosus (2001), J. Biol. Chem., 276, 45484-45490 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
ATP	presence of ATP stimulates in vitro replication of M13 ssDNA	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q9P9H1 and Q8U4H7	Q9P9H1, i.e. small subunit PriS, Q8U4H7, i.e. large subunit PriL	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
M13 ssDNA + n dNTP	-	Pyrococcus furiosus	M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
PriL	-	Pyrococcus furiosus
PriS	-	Pyrococcus furiosus

General Information

General Information	Comment	Organism
physiological function	the complex between subunits PriS and PriL shows higher DNA binding activity than the catalytic PriS subunit alone. The amount of DNA synthesized by the complex is much more abundant and shorter in length than that by PriS alone. The activity for RNA primer synthesis is not detected with PriS alone but observed using the complex in vitro	Pyrococcus furiosus

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Release 2023.1 (January 2023)