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Literature summary for 2.7.7.102 extracted from
- Properties of an unusual DNA primase from an archaeal plasmid (2007), Nucleic Acids Res., 35, 5635-5645.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, wild-type and mutant enzymes | Sulfolobus islandicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| delC370 | deletion mutant retains the strict ATP dependence for primer synthesis | Sulfolobus islandicus |
| delC526 | deletion mutant retains the strict ATP dependence for primer synthesis | Sulfolobus islandicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine 5'-(beta,gamma-imido)triphosphate | - |
Sulfolobus islandicus |  |
| adenosine 5'-(beta,gamma-methylenetriphosphate) | - |
Sulfolobus islandicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pH 7.5, 50°C, kinetic properties of the primase activity | Sulfolobus islandicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NTP + 7 dNTP | Sulfolobus islandicus | primases are specialized DNA-dependent RNA polymerases that synthesize a short oligoribonucleotide complementary to single-stranded template DNA. In the context of cellular DNA replication, primases are indispensable since DNA polymerases are not able to start DNA polymerization de novo | N(pdN)7 + 7 diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus islandicus | Q54324 | plasmid pRN1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus islandicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 7 dNTP | enzyme synthesizes short (8 nt-long) primers in the presence of single-stranded M13 DNA. The 12-bases-long substrate with the sequence 5'-CTTCTTCTGTGC-3' represents the minimal substrate which supports synthesis of the full-length primer. The base 3' to the central GTG motif is critical for primer synthesis and that the bases 5' of the GTG determine the length of the primer or run-off product but do not appear to be important for the efficiency of primer synthesis | Sulfolobus islandicus | A(pdN)7 + 7 diphosphate | - |
? | |
| NTP + 7 dNTP | primases are specialized DNA-dependent RNA polymerases that synthesize a short oligoribonucleotide complementary to single-stranded template DNA. In the context of cellular DNA replication, primases are indispensable since DNA polymerases are not able to start DNA polymerization de novo | Sulfolobus islandicus | N(pdN)7 + 7 diphosphate | - |
? | |
| NTP + 7 dNTP | the enzyme synthesizes a mixed primer consisting of a single ribonucleotide at the 5' end followed by seven deoxynucleotides. Ribonucleotides and deoxynucleotides are strictly required at the respective positions within the primer. The primase can initiate primer synthesis with an ATP regardless of the base 5' of the GTG motif and then proceed to synthesize a primer made up of dNTPs. The primase activity is highly sequence-specific and requires the trinucleotide motif GTG in the template. Primer synthesis starts outside of the recognition motif, immediately 5' to the recognition motif | Sulfolobus islandicus | N(pdN)7 + 7 diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| protein ORF904 | - |
Sulfolobus islandicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Sulfolobus islandicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pH 7.5, 50°C, kinetic properties of the primase activity | Sulfolobus islandicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Sulfolobus islandicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | primases are specialized DNA-dependent RNA polymerases that synthesize a short oligoribonucleotide complementary to single-stranded template DNA. In the context of cellular DNA replication, primases are indispensable since DNA polymerases are not able to start DNA polymerization de novo | Sulfolobus islandicus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pH 7.5, 50°C, kinetic properties of the primase activity | Sulfolobus islandicus |
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Release 2023.1 (January 2023)
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