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Literature summary for 2.7.7.101 extracted from
- Characterization of a functional DnaG-type primase in archaea: implications for a dual-primase system (2010), J. Mol. Biol., 397, 664-676.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E175Q | mutation drastically reduces priming activity, low level of DNA binding activity | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.085 | - |
NTP | KM-value is determined by measuring the priming rate at different concentrations of NTPs, 70°C, pH 8.5, 1 mM Mn2+, in presence of M13 ssDNA as template, wild-type enzyme | Saccharolobus solfataricus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | either Mg2+ or Mn2+ stimulates primer formation over that without any metals or Zn2+. A combination of both Mg2+ and Mn2+ does not stimulate further primer synthesis over that of the single metal Mn2+ | Saccharolobus solfataricus | |
| Mn2+ | either Mg2+ or Mn2+ stimulates primer formation over that without any metals or Zn2+. A combination of both Mg2+ and Mn2+ does not stimulate further primer synthesis over that of the single metal Mn2+ | Saccharolobus solfataricus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NTP + n NTP | Saccharolobus solfataricus | - |
N(pN)n + n diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | P95980 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NTP + n NTP | - |
Saccharolobus solfataricus | N(pN)n + n diphosphate | - |
? | |
| NTP + n NTP | SsoDnaG is only able to utilize NTPs and not dNTPs (in contrast to the heterodimeric eukaryotic DNA primases from archaea are able to incorporate either NTP or dNTP substrates to synthesize RNA, DNA, or hybrid primer products). Slightly positive cooperativity for binding at least two SsoDnaG molecules to DNA. SsoDnaG shows greater than fourfold faster rate of DNA priming over that of eukaryotic-type primase under optimal in vitro conditions. Incorporation of NTPs leads to the formation of primer products that ranged from 2 to 15 nucleotides. Dimer, 4 mer, and 13 mer products are primarily produced | Saccharolobus solfataricus | N(pN)n + n diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DnaG primase | - |
Saccharolobus solfataricus |
| protein SSO0079 | - |
Saccharolobus solfataricus |
| SsoDnaG | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 90 | 50°C: about 45% of maximal activity, 90°C: about 35% of maximal activity | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00055 | - |
NTP | KM-value is determined by measuring the priming rate at different concentrations of NTPs, 70°C, pH 8.5, in presence of M13 ssDNA as template, wild-type enzyme | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharolobus solfataricus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000033 | - |
NTP | KM-value is determined by measuring the priming rate at different concentrations of NTPs, 70°C, pH 8.5, in presence of M13 ssDNA as template, mutant enzyme E175Q | Saccharolobus solfataricus | |
| 0.0063 | - |
NTP | KM-value is determined by measuring the priming rate at different concentrations of NTPs, 70°C, pH 8.5, 1 mM Mn2+, in presence of M13 ssDNA as template, wild-type enzyme | Saccharolobus solfataricus | |
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