BRENDA - Enzyme Database
show all sequences of 2.7.7.10

Purification and properties of UDP-glucose (UDP-galactose) pyrophosphorylase from Bifidobacterium bifidum

Lee, L.J.; Kimura, A.; Tochikura, T.; J. Biochem. 86, 923-928 (1979)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
3-phosphoglycerate
slight activation
Bifidobacterium bifidum
D-erythrose 4-phosphate
slight activation
Bifidobacterium bifidum
D-fructose 6-phosphate
slight activation
Bifidobacterium bifidum
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibitors of UDP-galactose pyrophosphorolysis are alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate or galactose 6-phosphate
Bifidobacterium bifidum
UDP-galactose
UDP-glucose pyrophosphorolysis or UDP-galactose synthesis
Bifidobacterium bifidum
UDP-glucose
UDP-galactose pyrophosphorolysis
Bifidobacterium bifidum
UDP-xylose
UDP-galactose or UDP-glucose synthesis
Bifidobacterium bifidum
UTP
UDP-galactose pyrophosphorolysis
Bifidobacterium bifidum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
requirement
Bifidobacterium bifidum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
51000
-
4 * 51000, SDS-PAGE
Bifidobacterium bifidum
200000
-
gel filtration
Bifidobacterium bifidum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bifidobacterium bifidum
-
glucose-grown
-
Purification (Commentary)
Commentary
Organism
-
Bifidobacterium bifidum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diphosphate + UDP-xylose
at 10% the rate of UDP-glucose or UDP-galactose
642818
Bifidobacterium bifidum
?
-
-
-
r
additional information
no activity in the reverse reaction with UDP-N-acetylglucosamine, ADP-glucose, CDP-glucose, GDP-mannose or TDP-glucose
642818
Bifidobacterium bifidum
?
-
-
-
-
UTP + alpha-D-galactose 1-phosphate
reverse reaction at the same rate as UDP-glucose
642818
Bifidobacterium bifidum
diphosphate + UDP-galactose
-
642818
Bifidobacterium bifidum
r
UTP + alpha-D-glucose 1-phosphate
reverse reaction at the same rate as UDP-galactose
642818
Bifidobacterium bifidum
diphosphate + UDP-glucose
-
642818
Bifidobacterium bifidum
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 51000, SDS-PAGE
Bifidobacterium bifidum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bifidobacterium bifidum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7
broad
Bifidobacterium bifidum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
3-phosphoglycerate
slight activation
Bifidobacterium bifidum
D-erythrose 4-phosphate
slight activation
Bifidobacterium bifidum
D-fructose 6-phosphate
slight activation
Bifidobacterium bifidum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibitors of UDP-galactose pyrophosphorolysis are alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate or galactose 6-phosphate
Bifidobacterium bifidum
UDP-galactose
UDP-glucose pyrophosphorolysis or UDP-galactose synthesis
Bifidobacterium bifidum
UDP-glucose
UDP-galactose pyrophosphorolysis
Bifidobacterium bifidum
UDP-xylose
UDP-galactose or UDP-glucose synthesis
Bifidobacterium bifidum
UTP
UDP-galactose pyrophosphorolysis
Bifidobacterium bifidum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
requirement
Bifidobacterium bifidum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
51000
-
4 * 51000, SDS-PAGE
Bifidobacterium bifidum
200000
-
gel filtration
Bifidobacterium bifidum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bifidobacterium bifidum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diphosphate + UDP-xylose
at 10% the rate of UDP-glucose or UDP-galactose
642818
Bifidobacterium bifidum
?
-
-
-
r
additional information
no activity in the reverse reaction with UDP-N-acetylglucosamine, ADP-glucose, CDP-glucose, GDP-mannose or TDP-glucose
642818
Bifidobacterium bifidum
?
-
-
-
-
UTP + alpha-D-galactose 1-phosphate
reverse reaction at the same rate as UDP-glucose
642818
Bifidobacterium bifidum
diphosphate + UDP-galactose
-
642818
Bifidobacterium bifidum
r
UTP + alpha-D-glucose 1-phosphate
reverse reaction at the same rate as UDP-galactose
642818
Bifidobacterium bifidum
diphosphate + UDP-glucose
-
642818
Bifidobacterium bifidum
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 51000, SDS-PAGE
Bifidobacterium bifidum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bifidobacterium bifidum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7
broad
Bifidobacterium bifidum
Other publictions for EC 2.7.7.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697365
Karas-Kuzelicki
Synergistic effect of high lac ...
Homo sapiens
Clin. Biochem.
41
869-874
2008
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5
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2
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1
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-
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-
676036
Wehrli
Evidence for function of UDP g ...
Mus musculus, Mus musculus BALB/c
Mol. Genet. Metab.
91
191-194
2007
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-
-
-
1
-
-
-
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2
-
58
-
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1
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4
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1
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2
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1
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4
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673113
Mirzajani
The first study of galactose-1 ...
Homo sapiens
Clin. Biochem.
39
697-699
2006
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5
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2
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5
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1
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662883
Leslie
UDP-galactose pyrophosphorylas ...
Mus musculus
Mol. Genet. Metab.
85
21-27
2005
-
-
-
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2
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5
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1
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4
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2
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1
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4
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642821
Feusi
-
Galactosyl-sucrose metabolism ...
Cucumis melo, Glycine max
Physiol. Plant.
106
9-16
1999
-
-
-
-
-
-
1
-
-
-
2
3
-
2
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1
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3
3
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5
1
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1
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2
3
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1
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3
3
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5
1
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-
-
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-
-
-
642813
Lobelle-Rich
Separation and characterizatio ...
Entamoeba histolytica
Mol. Biochem. Parasitol.
7
173-182
1983
-
-
-
-
-
-
-
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1
1
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2
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1
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3
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1
1
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1
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3
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-
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-
-
-
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-
642816
Lobelle-Rich
-
Galactose-1-phosphate uridylyl ...
Entamoeba histolytica, Entamoeba histolytica K-9 / ATCC 30015
Methods Enzymol.
90
552-555
1982
-
-
-
-
-
-
-
3
-
2
1
2
-
3
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1
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1
1
8
-
1
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1
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3
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2
1
2
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1
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1
1
8
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1
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1
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-
642819
Smart
Separation and characteristics ...
Cucumis sativus
Planta
153
370-375
1981
1
-
-
-
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-
1
4
-
2
-
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2
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1
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2
3
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2
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1
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1
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2
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1
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1
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1
1
4
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2
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1
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2
3
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2
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1
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1
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2
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642818
Lee
Purification and properties of ...
Bifidobacterium bifidum
J. Biochem.
86
923-928
1979
3
-
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5
-
-
1
2
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1
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1
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4
1
1
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1
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3
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5
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1
2
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1
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4
1
1
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1
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642817
Lee
Presence of a single enzyme ca ...
Bifidobacterium bifidum
Biochim. Biophys. Acta
527
301-304
1978
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2
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1
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3
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1
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1
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3
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1
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3
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642814
Chacko
Uridine diphosphoglucose pyrop ...
Homo sapiens
Biochim. Biophys. Acta
268
113-120
1972
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4
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1
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2
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1
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2
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2
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1
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2
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1
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4
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1
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1
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2
2
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2
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1
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2
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1
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642815
Frydman
Thymidine diphosphate D-galact ...
Vigna radiata var. radiata
Biochim. Biophys. Acta
77
332-334
1963
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2
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642820
Pazur
Thymidine triphosphate: alpha- ...
Enterococcus faecalis
J. Biol. Chem.
238
3155-3160
1963
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2
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637084
Kalckar
The role of phosphoglycosyl co ...
Saccharomyces fragilis
Biochim. Biophys. Acta
12
250-264
1953
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