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Literature summary for 2.7.7.1 extracted from

  • Wang, X.; Zhou, Y.; Wang, L.; Liu, W.; Liu, Y.; Peng, C.; Zhao, Z.
    Engineering Escherichia coli nicotinic acid mononucleotide adenylyltransferase for fully active amidated NAD biosynthesis (2017), Appl. Environ. Microbiol., 83, e00692 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A86W/Y118N increase in activity with nicotinic acid ribonucleotide, and mutant accepts nicotinamide ribonucleotide as substrate Escherichia coli
Y84V/Y118D mutant prefers nicotinamide ribonucleotide over nicotinic acid ribonucleotide as substrate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
nicotinamide ribonucleotide mutant A86W/Y118N, pH 7.5, 30°C Escherichia coli
0.8
-
nicotinamide ribonucleotide mutant Y84V/Y118D, pH 7.5, 30°C Escherichia coli
9.4
-
nicotinamide ribonucleotide wild-type, pH 7.5, 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A140NE60 cf. EC 2.7.7.18
-
Escherichia coli BL21-DE3 A0A140NE60 cf. EC 2.7.7.18
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinamide ribonucleotide very poor substrate for wild-type Escherichia coli diphosphate + NAD+
-
?
ATP + nicotinamide ribonucleotide very poor substrate for wild-type Escherichia coli BL21-DE3 diphosphate + NAD+
-
?

Synonyms

Synonyms Comment Organism
nadD
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.76
-
nicotinamide ribonucleotide wild-type, pH 7.5, 30°C Escherichia coli
3 6 nicotinamide ribonucleotide mutant A86W/Y118N, pH 7.5, 30°C Escherichia coli
6.6
-
nicotinamide ribonucleotide mutant Y84V/Y118D, pH 7.5, 30°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.08
-
nicotinamide ribonucleotide wild-type, pH 7.5, 30°C Escherichia coli
8.3
-
nicotinamide ribonucleotide mutant Y84V/Y118D, pH 7.5, 30°C Escherichia coli
48
-
nicotinamide ribonucleotide mutant A86W/Y118N, pH 7.5, 30°C Escherichia coli