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Literature summary for 2.7.7.1 extracted from
- Identification of the nicotinamide mononucleotide adenylyltransferase of Trypanosoma cruzi (2015), Mem. Inst. Oswaldo Cruz, 110, 890-897.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene TcCLB.507047.170 or Tc00.1047053507047.170, DNA and amino acid sequence determination and analysis from genomic DNA, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Trypanosoma cruzi |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Trypanosoma cruzi |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + nicotinamide ribonucleotide | Trypanosoma cruzi | - |
diphosphate + NAD+ | - |
? | |
| ATP + nicotinamide ribonucleotide | Trypanosoma cruzi CL Brener | - |
diphosphate + NAD+ | - |
? | |
| additional information | Trypanosoma cruzi | bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 | ? | - |
? | |
| additional information | Trypanosoma cruzi CL Brener | bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | Q4DPR9 | gene TcCLB.507047.170 or Tc00.1047053507047.170 | - |
| Trypanosoma cruzi CL Brener | Q4DPR9 | gene TcCLB.507047.170 or Tc00.1047053507047.170 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + nicotinamide ribonucleotide | - |
Trypanosoma cruzi | diphosphate + NAD+ | - |
? | |
| ATP + nicotinamide ribonucleotide | - |
Trypanosoma cruzi CL Brener | diphosphate + NAD+ | - |
? | |
| additional information | bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 | Trypanosoma cruzi | ? | - |
? | |
| additional information | the enzyme shows activity for both NAMN and NMN, substrates used by NMNAT in the presence of ATP to form NAD+ and NAAD, respectively | Trypanosoma cruzi | ? | - |
? | |
| additional information | bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 | Trypanosoma cruzi CL Brener | ? | - |
? | |
| additional information | the enzyme shows activity for both NAMN and NMN, substrates used by NMNAT in the presence of ATP to form NAD+ and NAAD, respectively | Trypanosoma cruzi CL Brener | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme TcNMNAT secondary structure prediction and tertiary structure model, overview | Trypanosoma cruzi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nicotinamide mononucleotide adenylyltransferase | - |
Trypanosoma cruzi |
| NMNAT | - |
Trypanosoma cruzi |
| Tc00.1047053507047.170 | - |
Trypanosoma cruzi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Trypanosoma cruzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Trypanosoma cruzi |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | NAD+ biosynthesis is performed by de novo and salvage pathways, which converge on the step that is catalysed by the enzyme nicotinamide mononucleotide adenylyltransferase, NMNAT | Trypanosoma cruzi |
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Release 2023.1 (January 2023)
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