Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Methanothermobacter thermautotrophicus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, in 100 mM HEPES, pH 7.5 at 20°C, crystals of native enzyme were grown in 1.5 M Li2SO4, crystals of H19A enzyme were grown in 1.6 M Li2SO4, space group: P6322 | Methanothermobacter thermautotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
H19A | loss of activity | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Methanothermobacter thermautotrophicus | diphosphate + NAD+ | - |
r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Methanothermobacter thermautotrophicus | diphosphate + nicotinic acid-adenine dinucleotide | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | x-ray crystallography | Methanothermobacter thermautotrophicus |