Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.1 extracted from

  • Saridakis, V.; Christendat, D.; Kimber, M.S.; Dharamsi, A.; Edwards, A.M.; Pai, E.F.
    Insights into ligand binding and catalysis of a central step in NAD+ synthesis: structures of Methanobacterium thermoautotrophicum NMN adenylyltransferase complexes (2001), J. Biol. Chem., 276, 7225-7232.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Methanothermobacter thermautotrophicus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, in 100 mM HEPES, pH 7.5 at 20°C, crystals of native enzyme were grown in 1.5 M Li2SO4, crystals of H19A enzyme were grown in 1.6 M Li2SO4, space group: P6322 Methanothermobacter thermautotrophicus

Protein Variants

Protein Variants Comment Organism
H19A loss of activity Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Methanothermobacter thermautotrophicus diphosphate + NAD+
-
r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Methanothermobacter thermautotrophicus diphosphate + nicotinic acid-adenine dinucleotide
-
r

Subunits

Subunits Comment Organism
hexamer x-ray crystallography Methanothermobacter thermautotrophicus