Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | stimulates the catalytic activity of NMNAT-2 up to 30% | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, NMNAT-2 with a 6 x His-tag | Homo sapiens |
overexpression in HeLa and HEK293 cells as GFP-fusion protein | Homo sapiens |
General Stability | Organism |
---|---|
dithiothreitol stabalizes enzyme NMNAT-2 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
ATP | - |
Homo sapiens | |
0.036 | - |
ATP | - |
Homo sapiens | |
0.116 | - |
nicotinic acid mononucleotide | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
divalent cations | - |
Homo sapiens | |
Mg2+ | optimal concentration of NMNAT-2 is 0.3 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
gel filtration, recombinant NMNAT-2 with a 6 x His-tag | Homo sapiens |
34400 | - |
calculated from nucleic acid sequence, NMNAT-2 | Homo sapiens |
36800 | - |
1 * 36800, SDS-PAGE, recombinant NMNAT-2 with a 6 x His-tag | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | Homo sapiens | - |
diphosphate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme NMNAT-2 | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15 | - |
NMNAT-2 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | - |
Homo sapiens | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Homo sapiens | diphosphate + NAD+ | - |
r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Homo sapiens | diphosphate + nicotinic acid-adenine dinucleotide | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36800, SDS-PAGE, recombinant NMNAT-2 with a 6 x His-tag | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | recombinant NMNAT-2 | Homo sapiens |