Literature summary for 2.7.6.5 extracted from

Manav, M.; Beljantseva, J.; Bojer, M.; Tenson, T.; Ingmer, H.; Hauryliuk, V.; Brodersen, D.
Structural basis for (p)ppGpp synthesis by the Staphylococcus aureus small alarmone synthetase RelP (2018), J. Biol. Chem., 293, 3254-3264 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of RelP in pre-catalytic state (bound to GTP and adenosine 5'-(alpha,beta-methylene)triphosphate), and post-catalytic state (bound to pppGpp). RelP forms a tetramer, but unlike RelQ (SAS1), it is strongly inhibited by both pppGpp and ppGpp and is insensitive to inhibition by RNA. The active site pocket is formed mainly by the beta-strands and the loops beta1/alpha2,alpha3/beta2, and beta3/beta4 in addition to two small helices, alpha2 and alpha5. pppGpp adopts different conformations inside the two active sites (chains A and B) of the dimer	Staphylococcus aureus

Crystallization (Comment)

Organism

structures of RelP in pre-catalytic state (bound to GTP and adenosine 5'-(alpha,beta-methylene)triphosphate), and post-catalytic state (bound to pppGpp). RelP forms a tetramer, but unlike RelQ (SAS1), it is strongly inhibited by both pppGpp and ppGpp and is insensitive to inhibition by RNA. The active site pocket is formed mainly by the beta-strands and the loops beta1/alpha2,alpha3/beta2, and beta3/beta4 in addition to two small helices, alpha2 and alpha5. pppGpp adopts different conformations inside the two active sites (chains A and B) of the dimer

Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
guanosine 3'-diphosphate 5'-diphosphate	-	Staphylococcus aureus
guanosine 3'-diphosphate 5'-triphosphate	product inhibition	Staphylococcus aureus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	structure shows an iron-binding site at the dimer interface	Staphylococcus aureus
Zinc	structure shows a putative Zn2x04-binding site	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	W8TMV6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + GTP	-	Staphylococcus aureus	AMP + guanosine 3'-diphosphate 5'-triphosphate	-	?

Synonyms

Synonyms	Comment	Organism
(p)ppGpp synthetase	-	Staphylococcus aureus
RelP	-	Staphylococcus aureus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.045	-	37°C, pH not specified in the publication	Staphylococcus aureus	guanosine 3'-diphosphate 5'-diphosphate
0.098	-	37°C, pH not specified in the publication	Staphylococcus aureus	guanosine 3'-diphosphate 5'-triphosphate