Literature summary for 2.7.6.5 extracted from

Hogg, T.; Mechold, U.; Malke, H.; Cashel, M.; Hilgenfeld, R.
Conformational antagonism between opposing active sites in a bifunctional RelA/SpoT homolog modulates (p)ppGpp metabolism during the stringent response [corrected] (2004), Cell, 117, 57-68.

Search for more literature for 2.7.6.5

Application

Application	Comment	Organism
drug development	the structural knowledge of the self-regulatory mechanisms for controlling the opposing catalytic activities of RelSeq can be used to design specific inhibitors that interfere with either of the active sites and may have the potential of being developped into powerful antibacterial drugs	Streptococcus dysgalactiae

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the N-terminal catalytic fragment (residues 1-385) in Escherichia coli BL21	Streptococcus dysgalactiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
the crystallographic asymmetric unit contains two copies of RelSeq 1-385, two catalytic domains are clearly evident within each monomer, with the hydrolase (residues 5-159) and the synthetase (residues 176-371) domains joined by an overlapping central 3-helix bundle (residues 135-195)	Streptococcus dysgalactiae

Protein Variants

Protein Variants	Comment	Organism
D264G	eliminates detectable synthetase activity without appreciably altering the hydrolase activity	Streptococcus dysgalactiae
E323Q	eliminates detectable synthetase activity without appreciably altering the hydrolase activity	Streptococcus dysgalactiae

Inhibitors

Inhibitors	Comment	Organism	Structure
alpha,beta-methylene-ATP	-	Streptococcus dysgalactiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essential for activation, the enzyme has a single Mg2+ binding site	Streptococcus dysgalactiae
Mn2+	necessary cofactor at the hydrolase active site	Streptococcus dysgalactiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + GTP	Streptococcus dysgalactiae	bifunctional enzyme with synthetase and hydrolase activities	AMP + guanosine 5'-triphosphate 3'-diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Streptococcus dysgalactiae	-	subsp. equisimilis	-

Purification (Commentary)

Purification (Comment)	Organism
the catalytic fragment RelSeq 1-385	Streptococcus dysgalactiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + GTP	bifunctional enzyme with synthetase and hydrolase activities	Streptococcus dysgalactiae	AMP + guanosine 5'-triphosphate 3'-diphosphate	-	r

Synonyms

Synonyms	Comment	Organism
Rel/Spo protein	-	Streptococcus dysgalactiae
RelSeq	-	Streptococcus dysgalactiae

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Release 2023.1 (January 2023)