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Literature summary for 2.7.6.3 extracted from

  • Pemble, C.W.; Mehta, P.K.; Mehra, S.; Li, Z.; Nourse, A.; Lee, R.E.; White, S.W.
    Crystal structure of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase bifunctional enzyme from Francisella tularensis (2010), PLoS ONE, 5, e14165.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.6.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Francisella tularensis

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme and in complex with substrate 6-hydroxymethyl-7,8-dihydropteridine or 2-(7-amino-1-methyl-4,5-dioxo-1,4,5,6-tetrahydorpyrimido[4,5-c]pyridazin-3-yl)propanoic acid, sitting drop vapor diffusion method, using 90 mM Tris (pH 8.0), 190 mM sodium acetate, 24% (w/v) polyethylene glycol (PEG) 4000, and 17% (v/v) glycerol, at 18°C Francisella tularensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ contains two Mg2+ ions Francisella tularensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50509
-
 1 * 50509, the bifunctional enzyme HPPK-DHPS exists mainly as a monomer in solution, calculated from amino acid sequence Francisella tularensis
53000
-
 1 * 53000, the bifunctional enzyme HPPK-DHPS exists mainly as a monomer in solution, sedimentation velocity and equilibrium analysis Francisella tularensis

Organism

Organism UniProt Comment Textmining
Francisella tularensis A0A6B2JKF9
-
-
Francisella tularensis LVS A0A6B2JKF9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Francisella tularensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 6-hydroxymethyl-7,8-dihydropteridine
-
 Francisella tularensis AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
-
 ir
ATP + 6-hydroxymethyl-7,8-dihydropteridine
-
 Francisella tularensis LVS AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
-
 ir
additional information the enzyme does not hydrolyze alpha,beta-methyleneadenosine 5’-triphosphate Francisella tularensis ?
-
 ?
additional information the enzyme does not hydrolyze alpha,beta-methyleneadenosine 5’-triphosphate Francisella tularensis LVS ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50509, the bifunctional enzyme HPPK-DHPS exists mainly as a monomer in solution, calculated from amino acid sequence Francisella tularensis
monomer 1 * 53000, the bifunctional enzyme HPPK-DHPS exists mainly as a monomer in solution, sedimentation velocity and equilibrium analysis Francisella tularensis

Synonyms

Synonyms Comment Organism
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase HPPK-DHPS, bifunctional enzyme Francisella tularensis
HPPK
-
 Francisella tularensis