Literature summary for 2.7.6.3 extracted from

Li, Y.; Blaszczyk, J.; Wu, Y.; Shi, G.; Ji, X.; Yan, H.
Is the critical role of loop 3 of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase in catalysis due to loop-3 residues arginine-84 and tryptophan-89? Site-directed mutagenesis, biochemical, and crystallographic studies (2005), Biochemistry, 44, 8590-8599.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
apo W89A and its ternary complex with Mg-alpha,beta-methyleneadenosine triphosphate and 6-hydroxymethyl-7,8-dihydropterin are crystallized at 19°C using the hanging-drop vapor-diffusion technique. The structure of the ternary complex is determined at 1.25 A resolution	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
R84A	little changes in the dissociation constants and kinetic constants of the HPPK-catalyzed reaction	Escherichia coli
W89A	mutation increases the Kd for the binding of MgATP2- by a factor of 3, whereas the Kd for 6-hydroxymethyl-7,8-dihydropterin increases by a factor of 6, which is due to the increase in the dissociation rate constant. The mutation decreases the rate constant for the chemical step of the forward reaction by a factor of 15 and the rate constant for the chemical step of the reverse reaction by a factor of 25. The crystal structures of W89A show that W89A has different conformations in loops 2 and 3, but the critical catalytic residues are positioned for catalysis	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P26281	-	-

Purification (Commentary)

Purification (Comment)	Organism
HPPK-glutathione S-transferase fusion proteins	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 6-hydroxymethyl-7,8-dihydropteridine	-	Escherichia coli	AMP + 7,8-dihydro-6-(diphosphooxymethyl)pteridine	-	?

Synonyms

Synonyms	Comment	Organism
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase	-	Escherichia coli
HPPK	-	Escherichia coli

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Release 2023.1 (January 2023)