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Literature summary for 2.7.6.3 extracted from
- The hydroxymethyldihydropterin pyrophosphokinase domain of the multifunctional folic acid synthesis Fas protein of Pneumocystis carinii expressed as an independent enzyme in Escherichia coli: refolding and characterization of the recombinant enzyme (1994), Protein Expr. Purif., 5, 371-378.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the hydroxymethyldihydropterin pyrophosphokinase domain of the multifunctional folic acid synthesis Fas protein expressed as an independent enzyme in Escherichia coli, high level expression in inclusion bodies using an inducible tac promoter expression system | Pneumocystis carinii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0036 | - |
6-hydroxymethyl-7,8-dihydropteridine | pH 8.2, 50 mM Tris buffer | Pneumocystis carinii |  |
| 0.015 | - |
ATP | - |
Escherichia coli | |
| 0.015 | - |
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | less effective than Mn2+ or Mg2+ in activation | Escherichia coli | |
| Mg2+ | required, optimal activity at 4 mM | Escherichia coli | |
| Mn2+ | can replace Mg2+ with a 10 mM optimum | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Pneumocystis carinii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, enzyme loses activity over a period of months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine | - |
Escherichia coli | AMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine | - |
? | |
| ATP + 6-hydroxymethyl-7,8-dihydropteridine | - |
Pneumocystis carinii | AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate | - |
? | |
| dATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine | - |
Escherichia coli | dAMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase domain of the multifunctional folic acid synthesis enzyme | Pneumocystis carinii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase domain of the multifunctional folic acid synthesis enzyme | Pneumocystis carinii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
60 min, 75% loss of activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pneumocystis carinii |
| 8.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10.8 | active between pH 7.5 and pH 10.8 | Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pneumocystis carinii | isoelectric focusing | - |
9.1 |
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Release 2023.1 (January 2023)
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