Cloned (Comment) | Organism |
---|---|
gene CA1462, expression in Escherichia coli | Candida albicans |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complex with ATP analogue AMP-PNP and thiamine, hanging drop vapor diffusion method, mixing of 500 nl of protein solution with 500 nl of reservoir solution containing 17.5-20% PEG 4000, 0.2 M MgCl2 , 0.1 M Tris, and 20% glycerol, pH 7.0-pH 7.5, and equilibration against 1 ml or reservoir solution, X-ray diffraction structure determination and analysis at 1.96-2.1 A resolution | Candida albicans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | ligands electronic density map and Mg2+ coordination structure, the delta-oxygen from D113 and D115 and one oxygen atom from each phosphate group of the thiamine-PNP. The last coordination of this Mg2+ involves an oxygen atom from the free inorganic phosphate, overview | Candida albicans | |
additional information | ligand binding structures, overview | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant CA1462 from Escherichia coli | Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000367 | - |
recombinant enzyme | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | thiamine binding site structure, overview | Candida albicans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | three-dimensional enzyme structure, modelling, overview | Candida albicans |
Synonyms | Comment | Organism |
---|---|---|
CA1462 | - |
Candida albicans |
thiamine pyrophosphokinase | - |
Candida albicans |
TPK | - |
Candida albicans |