Literature summary for 2.7.6.1 extracted from

Andersen, R.W.; Leggio, L.L.; Hove-Jensen, B.; Kadziola, A.
Structure of dimeric, recombinant Sulfolobus solfataricus phosphoribosyl diphosphate synthase: a bent dimer defining the adenine specificity of the substrate ATP (2015), Extremophiles, 19, 407-415.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapour diffusion sitting or hanging drop method. The protein is co-crystallised in the presence of AMP and D-ribose 5-phosphate, but in the electron density map of the active site only AMP and a sulphate ion are observed. Sulphate ion, reminiscent of the ammonium sulfate precipitation step of the purification, seems to bind tightly and, therefore, presumably occupies and blocks the D-ribose 5-phosphate binding site. The structure is determined at 2.8 A resolution	Saccharolobus solfataricus

Crystallization (Comment)

Organism

vapour diffusion sitting or hanging drop method. The protein is co-crystallised in the presence of AMP and D-ribose 5-phosphate, but in the electron density map of the active site only AMP and a sulphate ion are observed. Sulphate ion, reminiscent of the ammonium sulfate precipitation step of the purification, seems to bind tightly and, therefore, presumably occupies and blocks the D-ribose 5-phosphate binding site. The structure is determined at 2.8 A resolution

Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	2 * 34000, SDS-PAGE	Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + D-ribose 5-phosphate	Saccharolobus solfataricus	-	AMP + 5-phospho-alpha-D-ribose 1-diphosphate	-	?
ATP + D-ribose 5-phosphate	Saccharolobus solfataricus P2	-	AMP + 5-phospho-alpha-D-ribose 1-diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97Z86	-	-
Saccharolobus solfataricus P2	Q97Z86	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-ribose 5-phosphate	-	Saccharolobus solfataricus	AMP + 5-phospho-alpha-D-ribose 1-diphosphate	-	?
ATP + D-ribose 5-phosphate	-	Saccharolobus solfataricus P2	AMP + 5-phospho-alpha-D-ribose 1-diphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 34000, SDS-PAGE	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
5-phosphoribosyl-1-alpha-diphosphate synthase	-	Saccharolobus solfataricus
PRPP synthase	-	Saccharolobus solfataricus
SSO1045	locus name	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Saccharolobus solfataricus