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Literature summary for 2.7.6.1 extracted from
- Biochemical characterization of the Mycobacterium tuberculosis phosphoribosyl-1-pyrophosphate synthetase (2011), Glycobiology, 21, 410-425.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | the enzyme requires the presence of phosphate at a concentration of 50 mM for full activation | Mycobacterium tuberculosis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli C41 cells | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | strong inhibitor | Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0082 | - |
D-ribose 5-phosphate | at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Mycobacterium tuberculosis | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mycobacterium tuberculosis | |
| Mn2+ | required | Mycobacterium tuberculosis | |
| additional information | Mn2+ and Mg2+ cannot be replaced by Ca2+ | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA agarose column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-adenylyl-beta,gamma-imidodiphosphate + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 5'-adenylyl-beta,gamma-imidodiphosphate + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| ATP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
ir | |
| ATP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis H37Rv | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | - |
Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphoribosyl-1-pyrophosphate synthetase | - |
Mycobacterium tuberculosis |
| PRPP synthetase | - |
Mycobacterium tuberculosis |
| PrsA | - |
Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Mycobacterium tuberculosis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | the enzyme shows activity across a broad range of pH levels (4.5-9.5) and is only found to be inactive at high pH 10.5 | Mycobacterium tuberculosis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.522 | - |
ADP | at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7430 | - |
D-ribose 5-phosphate | at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
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