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Literature summary for 2.7.6.1 extracted from

  • Gallois, R.; Prevot, J.C.; Clement, A.; Jacob, J.L.
    Purification and characterization of phosphoribosylpyrophosphate synthetase from rubber tree latex (1997), Plant Physiol., 115, 847-852.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
DTT requirement Hevea brasiliensis
additional information no other sulfhydryl group protector or antioxidant can effectively replace DTT Hevea brasiliensis
phosphate activation at 1 mM of D-ribose 5-phosphate, maximal activity from 10 mM of D-ribose 5-phosphate Hevea brasiliensis
sulfate no activity observed Hevea brasiliensis

Inhibitors

Inhibitors Comment Organism Structure
5-phospho-alpha-D-ribose 1-diphosphate
-
Hevea brasiliensis
adenosine no inhibition Hevea brasiliensis
ADP no inhibition Hevea brasiliensis
AMP no inhibition Hevea brasiliensis
ATP no inhibition Hevea brasiliensis
D-fructose 1,6-diphosphate no inhibition Hevea brasiliensis
GDP no inhibition Hevea brasiliensis
glyceraldehyde 3-phosphate no inhibition Hevea brasiliensis
GMP no inhibition Hevea brasiliensis
GTP no inhibition Hevea brasiliensis
guanosine no inhibition Hevea brasiliensis
L-histidine no inhibition Hevea brasiliensis
L-tryptophan no inhibition Hevea brasiliensis
Mn2+ complete inhibition above 0.4 mM Hevea brasiliensis
additional information D-glucose-6-phosphate have no effects on the enzyme activity; dihydroxyacetone phosphate, D-glucose-1-phosphate, fructose-6-phosphate, ribose, sucrose, glutamine, glutamic acid, aspartic acid, alanine, alpha-ketoglutarate, pyruvate, citrate, malate, fumarate, succinate, cytidine, uridine, adenine, orotate, guanine, cytosine and xanthine have no effects on the enzyme activity Hevea brasiliensis
phosphate inhibition at low concentrations of D-ribose 5-phosphate Hevea brasiliensis
UDP no inhibition Hevea brasiliensis
UMP no inhibition Hevea brasiliensis
UTP no inhibition Hevea brasiliensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
D-ribose 5-phosphate pH 7.5, 37ºC Hevea brasiliensis
0.2
-
ATP pH 7.5, 37ºC Hevea brasiliensis
1.5
-
Mg2+ pH 7.5, 37ºC Hevea brasiliensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol also known as latex in specialized cells Hevea brasiliensis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ no activity observed Hevea brasiliensis
Mg2+ requirement Hevea brasiliensis
Mn2+ no activity observed Hevea brasiliensis
additional information K+, Na+, Fe2+ Cu2+ and Ca2+ have no notable effects on the enzyme activity Hevea brasiliensis
Zn2+ no activity observed Hevea brasiliensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
x * 57000, SDS-PAGE Hevea brasiliensis
200000
-
gel filtration Hevea brasiliensis

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis
-
rubber tree
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, chromatography on Sephacryl S300 HR and on Protein-Pak DEAE 40 HR and affinity chromatography Hevea brasiliensis

Source Tissue

Source Tissue Comment Organism Textmining
laticifer
-
Hevea brasiliensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
Hevea brasiliensis AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
CTP + D-ribose 5-phosphate does not serve as substrate Hevea brasiliensis CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
GTP + D-ribose 5-phosphate does not serve as substrate Hevea brasiliensis GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
ITP + D-ribose 5-phosphate does not serve as substrate Hevea brasiliensis IMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
UTP + D-ribose 5-phosphate does not serve as substrate Hevea brasiliensis UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Hevea brasiliensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Hevea brasiliensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Hevea brasiliensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
Mn2+ pH 7.5, 37ºC Hevea brasiliensis
0.03
-
5-phospho-alpha-D-ribose 1-diphosphate pH 7.5, 37ºC Hevea brasiliensis