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Literature summary for 2.7.6.1 extracted from

  • Ishijima, S.; Asai, T.; Kita, K.; Sonoda, T.; Tatibana, M.
    Partial reconstitution of mammalian phosphoribosylpyrophosphate synthetase in Escherichia coli cells. Coexpression of catalytic subunits with the 39-kDa associated protein leads to formation of soluble multimeric complexes of various compositions (1997), Biochim. Biophys. Acta, 1342, 28-36.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of isoenzymes PRSI and PRSII in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ADP when PAP39 complexes with PRSI or PRSII, the sensitivity to ADP inhibition is lowered Rattus norvegicus
GDP when PAP39 complexes with PRSI or PRSII, the sensitivity to ADP inhibition is lowered Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
the catalytic subunit complexes with the PRPP synthetase-associated protein of 39000 Da, PAP39, to form highly aggregated forms Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-ribose 5-phosphate Rattus norvegicus the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
rat
-

Purification (Commentary)

Purification (Comment) Organism
polyethylene glycol and acid precipitation Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the specific activity of the catalytic subunit is lower in the complexes where the amount of PRPP synthetase-associated protein of 39000 Da, PAP39, is higher. Increasing amounts of PAP39 have strong inhibitory effects on the catalytic activity Rattus norvegicus
10.4
-
liver enzyme complex with PAP39 and PRS at a mass ratio of 0.34 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r