Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | the enzyme in MAU V cells is less sensitive than the enzyme in extracts of wild-type cells | Rattus norvegicus | |
AMP | the enzyme in MAU V cells is less sensitive than the enzyme in extracts of wild-type cells, competitive with respect to ATP | Rattus norvegicus | |
nucleotides | enzyme from HTC cells has an altered sensitivity to feedback inhibition by purine and pyrimidine nucleotides | Rattus norvegicus | |
TDP | the enzyme in MAU V cells is less sensitive than the enzyme in extracts of wild-type cells, competitive with respect to ATP | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
phosphate | pH 7.4, 37ºC | Rattus norvegicus | |
0.045 | - |
ATP | pH 7.4, 37ºC | Rattus norvegicus | |
0.2 | - |
D-ribose 5-phosphate | pH 7.4, 37ºC | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HTC cell | cell line of rat hepatoma cells in continuous culture, clone MAU V with an inreased ability to salvage exogenous purines | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
ADP | pH 7.4, 37ºC, wild-type cells | Rattus norvegicus | |
0.01 | - |
AMP | pH 7.4, 37ºC, wild-type cells | Rattus norvegicus | |
0.1 | - |
AMP | pH 7.4, 37ºC, MAU V cells | Rattus norvegicus | |
0.15 | - |
TDP | pH 7.4, 37ºC, wild-type cells | Rattus norvegicus | |
0.175 | - |
ADP | pH 7.4, 37ºC, MAU V cells | Rattus norvegicus | |
0.75 | - |
TDP | pH 7.4, 37ºC, MAU V cells | Rattus norvegicus |