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Literature summary for 2.7.6.1 extracted from

  • Green, C.D.; Martin, D.W.
    Characterization of a feedback-resistant phosphoribosylpyrophosphate synthetase from cultured, mutagenized hepatoma cells that overproduce purines (1973), Proc. Natl. Acad. Sci. USA, 70, 3698-3702.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
ADP the enzyme in MAU V cells is less sensitive than the enzyme in extracts of wild-type cells Rattus norvegicus
AMP the enzyme in MAU V cells is less sensitive than the enzyme in extracts of wild-type cells, competitive with respect to ATP Rattus norvegicus
nucleotides enzyme from HTC cells has an altered sensitivity to feedback inhibition by purine and pyrimidine nucleotides Rattus norvegicus
TDP the enzyme in MAU V cells is less sensitive than the enzyme in extracts of wild-type cells, competitive with respect to ATP Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
phosphate pH 7.4, 37ºC Rattus norvegicus
0.045
-
ATP pH 7.4, 37ºC Rattus norvegicus
0.2
-
D-ribose 5-phosphate pH 7.4, 37ºC Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
rat
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
HTC cell cell line of rat hepatoma cells in continuous culture, clone MAU V with an inreased ability to salvage exogenous purines Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.008
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
ADP pH 7.4, 37ºC, wild-type cells Rattus norvegicus
0.01
-
AMP pH 7.4, 37ºC, wild-type cells Rattus norvegicus
0.1
-
AMP pH 7.4, 37ºC, MAU V cells Rattus norvegicus
0.15
-
TDP pH 7.4, 37ºC, wild-type cells Rattus norvegicus
0.175
-
ADP pH 7.4, 37ºC, MAU V cells Rattus norvegicus
0.75
-
TDP pH 7.4, 37ºC, MAU V cells Rattus norvegicus