Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | requirement | Homo sapiens | |
phosphate | specific and absolute requirement | Homo sapiens | |
phosphate | no inhibition up to 75 mM | Homo sapiens | |
sulfate | can partially replace phosphate in activation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of isoenzymes PRSI and PRSII in Escherichia coli | Homo sapiens |
General Stability | Organism |
---|---|
dilution of highly purified isoenzyme PRSII to 0.1 mg/ml lacking Mg2+ and ATP results in immediate loss of 65% activity and 88% activity after 3 h at 4ºC. Dilution of isoenzyme PRSI to 0.1 mg/ml lacking Mg2+ and ATP results in loss of 10% activity after 3h at 4ºC | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-diphosphoglycerate | at 1 mM, inhibition of isoenzyme PRSI and PRSII, at 1-10 mM, stimulation of isoenzyme PRSI | Homo sapiens | |
ADP | the most effective | Homo sapiens | |
Ca2+ | - |
Homo sapiens | |
CDP | no effect at 1 mM | Homo sapiens | |
CTP | no effect at 1 mM | Homo sapiens | |
GDP | - |
Homo sapiens | |
GMP | no effect at 1 mM | Homo sapiens | |
UDP | no effect at 1 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
ATP | pH 7.5, 37ºC, isoenzyme PRSI | Homo sapiens | |
0.021 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
0.052 | - |
D-ribose 5-phosphate | pH 7.5, 37ºC, isoenzyme PRSI | Homo sapiens | |
0.07 | - |
Mg2+ | pH 7.5, 37ºC, isoenzyme PRSI | Homo sapiens | |
0.083 | - |
D-ribose 5-phosphate | pH 7.5, 37ºC, isoenzyme PRSII | Homo sapiens | |
0.11 | - |
Mg2+ | pH 7.5, 37ºC, isoenzyme PRSII | Homo sapiens | |
0.7 | - |
phosphate | pH 7.5, 37ºC, isoenzyme PRSI | Homo sapiens | |
2.1 | - |
phosphate | pH 7.5, 37ºC, isoenzyme PRSII | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Homo sapiens | |
Mg2+ | the most effective | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
1000000 | - |
gel filtration, isoenzymes PRSI and PRSII | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Homo sapiens | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
streptomycin precipitation and ammonium sulfate fractionation for isoenzyme PRSI and polyethylene glycol, acid and ammonium sulfate precipitation for isoenzyme PRSII | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
isoenzyme PRSI | Homo sapiens |
35.7 | - |
isoenzyme PRSII | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
isoenzyme PRSII is more thermolabile than PRSI. After 10 min, isoenzyme PRSI loses about 30% of activity, isoenzyme PRSII, about 85% of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
isoenzyme PRSII | Homo sapiens |
7.5 | - |
at 40-50 mM phosphate, isoenzymes PRSI and PRSII | Homo sapiens |
8.8 | - |
isoenzyme PRSI | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.3 | At pH 6.5, about 25% and 50% of maximum activity for isoenzymes PRSI and PRSII, respectively. At pH 9.3, about 40% and 60% of maximum activity for isoenzymes PRSII and PRSI, respectively | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
85% of maximal activity, isoenzyme PRSI | Homo sapiens |
8.8 | - |
41% of maximal activity, isoenzyme PRSII | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | two-dimensional protein electrophoresis, isoenzyme PRSI | - |
5.5 |
Homo sapiens | two-dimensional protein electrophoresis, isoenzyme PRSII | - |
5.7 |