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Literature summary for 2.7.6.1 extracted from

  • Fox, I.H.; Kelley, W.N.
    Human phosphoribosylpyrophosphate synthetase. Kinetic mechanism and end product inhibition (1972), J. Biol. Chem., 247, 2126-2131.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate
-
Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2,3-diphosphoglycerate 30% inhibition at 1 mM; competitive to D-ribose 5-phosphate Homo sapiens
2-deoxy-D-ribose 5-phosphate 92% inhibition at 1 mM Homo sapiens
5-phospho-alpha-D-ribose 1-diphosphate competitive to ribose 5-phosphate Homo sapiens
adenosine 27% inhibition at 1 mM Homo sapiens
ADP 99% inhibition at 1 mM; competitive inhibition to ATP; end product inhibition; the most effective Homo sapiens
allopurinol ribonucleotides 23% inhibition at 1 mM Homo sapiens
AMP 69% inhibition at 1 mM Homo sapiens
CDP 44% inhibition at 1 mM Homo sapiens
CTP 40% inhibition at 1 mM Homo sapiens
D-fructose 1,6-diphosphate 20% inhibition at 1 mM Homo sapiens
D-Fructose 1-phosphate 19% inhibition at 1 mM Homo sapiens
D-ribose 1-phosphate 13% inhibition at 1 mM Homo sapiens
FAD 51% inhibition at 1 mM Homo sapiens
GDP
-
Homo sapiens
glyceraldehyde 3-phosphate 14% inhibition at 1 mM Homo sapiens
GMP 27% inhibition at 1 mM Homo sapiens
GTP 51% inhibition at 1 mM Homo sapiens
guanosine 20% inhibition at 1 mM Homo sapiens
IDP 47% inhibition at 1 mM Homo sapiens
IMP 21% inhibition at 1 mM Homo sapiens
ITP 36% inhibition at 1 mM Homo sapiens
additional information inhibition by pair of inhibitors tested is not greater than the sum of the inhibition produced by each alone Homo sapiens
NAD+ 100% inhibition at 1 mM Homo sapiens
NADH 72% inhibition at 1 mM Homo sapiens
NADPH 100% inhibition at 1 mM Homo sapiens
TDP 100% inhibition at 1 mM Homo sapiens
TTP 47% inhibition at 1 mM Homo sapiens
UDP 10%-16% inhibition at 1 mM Homo sapiens
UMP 10%-18% inhibition at 1 mM Homo sapiens
XDP 36% inhibition at 1 mM Homo sapiens
XTP 22% inhibition at 1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
Mg2+ pH 7.4, 37ºC Homo sapiens
0.014
-
ATP pH 7.4, 37ºC Homo sapiens
0.014
-
ATP ATP in form of MgATP2- Homo sapiens
0.033
-
ribose 5-phosphate pH 7.4, 37ºC Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-ribose 5-phosphate Homo sapiens the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 400fold purification Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction with ribose-5-phosphate binding first and 5-phosphoribose diphosphate being released last Homo sapiens
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 37ºC Homo sapiens
0.3
-
AMP pH 7.4, 37ºC, with D-ribose-5-phosphate Homo sapiens
0.4
-
AMP pH 7.4, 37ºC, with MgATP2- Homo sapiens