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Literature summary for 2.7.6.1 extracted from
- Ribosephosphate pyrophosphokinase (rat liver) (1978), Methods Enzymol., 51, 12-17.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | can partially replace phosphate in activation | Rattus norvegicus |  |
| phosphate | requirement | Rattus norvegicus | |
| sulfate | can partially replace phosphate in activation | Rattus norvegicus | |
General Stability
| General Stability | Organism |
|---|---|
| high enzyme concentration, albumin, 0.05 mg/ml, EDTA, 1 mM or DTT, 1 mM stabilizes during assay | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive inhibition to ATP | Rattus norvegicus | |
| AMP | noncompetitive to both substrates | Rattus norvegicus | |
| ATP | free form, slight inhibition at 1 mM | Rattus norvegicus | |
| D-ribose 5-phosphate | substrate inhibition above 1.5 mM | Rattus norvegicus | |
| dADP | competitive to ATP | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
ATP | pH and temperature conditions not mentioned | Rattus norvegicus | |
| 0.22 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
| 0.29 | - |
5-phospho-D-ribose diphosphate | pH and temperature conditions not mentioned | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required to form a complex with ATP and as a free cation | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
purified enzyme is in an aggregated state | Rattus norvegicus |
| 40000 | - |
x * 40000, SDS-PAGE with mercaptoethanol | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose 5-phosphate | Rattus norvegicus | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetone powder, acid and streptomycin precipitation, heat treatment and agarose chromatography, 1500 to 3000fold purification | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.05 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50% loss of activity after 2 months. -20ºC, rapid loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose 5-phosphate | highly specific for D-ribose 5-phosphate | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| dATP + D-ribose 5-phosphate | - |
Rattus norvegicus | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE with mercaptoethanol | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.8 | - |
Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
ADP | pH and temperature conditions not mentioned | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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