Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can used to replenish ATP in ATP-consuming biochemical reactions | Cupriavidus necator |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109 | Cupriavidus necator |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent cation required for activity. 5 mM CaCl2 show 48% of the activity compared to 5 mM MnCl2 | Cupriavidus necator | |
Mg2+ | divalent cation required for activity. 5 mM MgCl2 show 84% of the activity compared to 5 mM MnCl2 | Cupriavidus necator | |
Mn2+ | divalent cation required for activity. Highest activity with 5 mM MnCl2 | Cupriavidus necator | |
Zn2+ | divalent cation required for activity. 5 mM ZnCl2 show 21% of the activity compared to 5 mM MnCl2 | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (phosphate)n+1 | about 80% compared to the activity with UDP. Additional formation of adenosine tetraphosphate | Cupriavidus necator | ATP + (phosphate)n | - |
r | |
ATP + (phosphate)n | 98% compared to the activity with CTP | Cupriavidus necator | ADP + (phosphate)n+1 | - |
r | |
CDP + (phosphate)n+1 | about 80% compared to the activity with UDP. Additional formation of cytidine tetraphosphate | Cupriavidus necator | CTP + (phosphate)n | - |
r | |
CTP + (phosphate)n | - |
Cupriavidus necator | CDP + (phosphate)n+1 | - |
r | |
dTDP + (phosphate)n+1 | about 80% compared to the activity with UDP. Additional formation of deoxythymidine tetraphosphate | Cupriavidus necator | dTTP + (phosphate)n | - |
r | |
dTTP + (phosphate)n | 98% compared to the activity with CTP | Cupriavidus necator | dTDP + (phosphate)n+1 | - |
r | |
GDP + (phosphate)n+1 | about 95% compared to the activity with UDP. Additional formation of guanidine tetraphosphate | Cupriavidus necator | GTP + (phosphate)n | - |
r | |
GTP + (phosphate)n | about 90% compared to the activity with CTP | Cupriavidus necator | GDP + (phosphate)n+1 | - |
r | |
additional information | thiamine pyrophosphate is not used as a substrate by PPK2c | Cupriavidus necator | ? | - |
- |
|
UDP + (phosphate)n+1 | additional formation of uridine tetraphosphate | Cupriavidus necator | UTP + (phosphate)n | - |
r | |
UTP + (phosphate)n | about 90% compared to the activity with CTP | Cupriavidus necator | UDP + (phosphate)n+1 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PPK2c | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 40 | - |
Cupriavidus necator |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | 10°C: about 55% of maximal activity, 28°C-40°C: maximal activity, 45°C: less than 5% of maximal activcity | Cupriavidus necator |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
rapid inactivation above | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Cupriavidus necator |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | pH 6.5: about 85% of maximal activity, pH 10.5: about 70% of maximal activity | Cupriavidus necator |