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Literature summary for 2.7.4.B7 extracted from

  • Hildenbrand, J.C.; Teleki, A.; Jendrossek, D.
    A universal polyphosphate kinase PPK2c of Ralstonia eutropha accepts purine and pyrimidine nucleotides including uridine diphosphate (2020), Appl. Microbiol. Biotechnol., 104, 6659-6667 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the enzyme can used to replenish ATP in ATP-consuming biochemical reactions Cupriavidus necator

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli JM109 Cupriavidus necator

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation required for activity. 5 mM CaCl2 show 48% of the activity compared to 5 mM MnCl2 Cupriavidus necator
Mg2+ divalent cation required for activity. 5 mM MgCl2 show 84% of the activity compared to 5 mM MnCl2 Cupriavidus necator
Mn2+ divalent cation required for activity. Highest activity with 5 mM MnCl2 Cupriavidus necator
Zn2+ divalent cation required for activity. 5 mM ZnCl2 show 21% of the activity compared to 5 mM MnCl2 Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)n+1 about 80% compared to the activity with UDP. Additional formation of adenosine tetraphosphate Cupriavidus necator ATP + (phosphate)n
-
r
ATP + (phosphate)n 98% compared to the activity with CTP Cupriavidus necator ADP + (phosphate)n+1
-
r
CDP + (phosphate)n+1 about 80% compared to the activity with UDP. Additional formation of cytidine tetraphosphate Cupriavidus necator CTP + (phosphate)n
-
r
CTP + (phosphate)n
-
Cupriavidus necator CDP + (phosphate)n+1
-
r
dTDP + (phosphate)n+1 about 80% compared to the activity with UDP. Additional formation of deoxythymidine tetraphosphate Cupriavidus necator dTTP + (phosphate)n
-
r
dTTP + (phosphate)n 98% compared to the activity with CTP Cupriavidus necator dTDP + (phosphate)n+1
-
r
GDP + (phosphate)n+1 about 95% compared to the activity with UDP. Additional formation of guanidine tetraphosphate Cupriavidus necator GTP + (phosphate)n
-
r
GTP + (phosphate)n about 90% compared to the activity with CTP Cupriavidus necator GDP + (phosphate)n+1
-
r
additional information thiamine pyrophosphate is not used as a substrate by PPK2c Cupriavidus necator ?
-
-
UDP + (phosphate)n+1 additional formation of uridine tetraphosphate Cupriavidus necator UTP + (phosphate)n
-
r
UTP + (phosphate)n about 90% compared to the activity with CTP Cupriavidus necator UDP + (phosphate)n+1
-
r

Synonyms

Synonyms Comment Organism
PPK2c
-
Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28 40
-
Cupriavidus necator

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 10°C: about 55% of maximal activity, 28°C-40°C: maximal activity, 45°C: less than 5% of maximal activcity Cupriavidus necator

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
rapid inactivation above Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10.5 pH 6.5: about 85% of maximal activity, pH 10.5: about 70% of maximal activity Cupriavidus necator