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Literature summary for 2.7.4.9 extracted from

  • Fucci, I.J.; Sinha, K.; Rule, G.S.
    Stabilization of active site dynamics leads to increased activity with 3-azido-3-deoxythymidine monophosphate for F105Y mutant human thymidylate kinase (2020), ACS Omega, 5, 2355-2367 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.9

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallographic structures of the mutant F105Y enzyme in complex with either TMP or 3'-azido-3'-deoxythymidine monophosphate show that the mutation shifts the P-loop to a position that is similar to that found in the complex between Ap5dT and the wild-type enzyme Homo sapiens

Protein Variants

Protein Variants Comment Organism
F105Y stabilization of active site dynamics leads to increased activity with 3-azido-3-deoxythymidine monophosphate for the mutant enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0038
-
 3'-azido-3'-deoxythymidine monophosphate pH and temperature not specified in the publication, mutant enzyme F105Y Homo sapiens
0.0042
-
 TMP pH and temperature not specified in the publication, mutant enzyme F105Y Homo sapiens
0.0063
-
 TMP pH and temperature not specified in the publication, wild-type enzyme Homo sapiens
0.012
-
 3'-azido-3'-deoxythymidine monophosphate pH and temperature not specified in the publication, wild-type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P23919
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3'-azido-3'-deoxythymidine monophosphate
-
 Homo sapiens ADP + 3'-azido-3'-deoxythymidine diphosphate
-
 ?
ATP + TMP
-
 Homo sapiens ADP + TDP
-
 ?

Synonyms

Synonyms Comment Organism
thymidylate kinase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 3'-azido-3'-deoxythymidine monophosphate pH and temperature not specified in the publication, wild-type enzyme Homo sapiens
0.17
-
 TMP pH and temperature not specified in the publication, mutant enzyme F105Y Homo sapiens
0.25
-
 3'-azido-3'-deoxythymidine monophosphate pH and temperature not specified in the publication, mutant enzyme F105Y Homo sapiens
0.73
-
 TMP pH and temperature not specified in the publication, wild-type enzyme Homo sapiens