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Literature summary for 2.7.4.9 extracted from
- Crystal structure of poxvirus thymidylate kinase: an unexpected dimerization has implications for antiviral therapy (2008), Proc. Natl. Acad. Sci. USA, 105, 16900-16905.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| TMPK bound to TDP and to brivudin monophosphate, hanging drop vapor diffusion method | Vaccinia virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (E)-5-(2-bromovinyl)-deoxyuridine | - |
Vaccinia virus |  |
| 5-bromo-deoxyuridine | - |
Vaccinia virus | |
| 5-chloro-deoxyuridine | - |
Vaccinia virus | |
| 5-fluoro-deoxyuridine | - |
Vaccinia virus | |
| 5-iodo-deoxyuridine | - |
Vaccinia virus | |
| deoxythymidine | - |
Vaccinia virus | |
| deoxyuridine | - |
Vaccinia virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
(E)-5-(2-bromovinyl)-deoxyuridine monophosphate | - |
Vaccinia virus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | contains Mg2+ at the active site | Vaccinia virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vaccinia virus | P68693 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (E)-5-(2-bromovinyl)-deoxyuridine monophosphate | 5% of the TMP efficiency | Vaccinia virus | ? | - |
? | |
| ATP + brivudin | - |
Vaccinia virus | ? | - |
? | |
| ATP + dGMP | 5% of the TMP efficiency | Vaccinia virus | ? | - |
? | |
| ATP + dUMP | - |
Vaccinia virus | ? | - |
? | |
| ATP + TMP | - |
Vaccinia virus | ? | - |
? | |
| N-methanocarbathymidine | - |
Vaccinia virus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | x-ray crystallography | Vaccinia virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thymidylate kinase | - |
Vaccinia virus |
| TMPK | - |
Vaccinia virus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42.5 | - |
the midtemperature of thermal denaturation is 42.5°C | Vaccinia virus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.86 | - |
(E)-5-(2-bromovinyl)-deoxyuridine monophosphate | - |
Vaccinia virus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Vaccinia virus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
5-bromo-deoxyuridine | - |
Vaccinia virus | |
| 0.015 | - |
5-chloro-deoxyuridine | - |
Vaccinia virus | |
| 0.023 | - |
5-iodo-deoxyuridine | - |
Vaccinia virus | |
| 0.025 | - |
deoxythymidine | - |
Vaccinia virus | |
| 0.5 | - |
(E)-5-(2-bromovinyl)-deoxyuridine | - |
Vaccinia virus | |
| 0.7 | - |
5-fluoro-deoxyuridine | - |
Vaccinia virus | |
| 1 | - |
deoxyuridine | - |
Vaccinia virus | |
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