Crystallization (Comment) | Organism |
---|---|
structural model based on staphylococcus aureus structure | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',3'-dideoxy-2',3'-didehydrothymidine | poor inhibitor | Bacillus anthracis | |
3'-azidothymidine | poor inhibitor | Bacillus anthracis | |
5-fluorodeoxyuridine | poor inhibitor | Bacillus anthracis | |
D-2'-fluoroarabinosyl-5-methyldeoxyuridine | poor inhibitor; strong | Bacillus anthracis | |
deoxythymidine | poor inhibitor | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate | pH 7.6, 37°C | Bacillus anthracis | |
0.033 | - |
dTMP | - |
Bacillus anthracis | |
0.167 | - |
L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate | pH 7.6, 37°C | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q81W11 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + dTMP = ADP + dTDP | binding of D-dTMP is through a network of hydrogen bonds to the pyrimidine ring involving residues R70, S97, and Q101, and the base is stacked by residue Y66. The phosphate group is tightly coordinated by residues R34, D91, and R92 | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-monophosphate | - |
Bacillus anthracis | ADP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-diphosphate | - |
? | |
ATP + 5-fluorodeoxyuridine 5'-monophosphate | poor substrate | Bacillus anthracis | ADP + 5-fluorodeoxyuridine 5'-diphosphate | - |
? | |
ATP + AMP | 3.7% of the activity with dTMP | Bacillus anthracis | ADP + ADP | - |
? | |
ATP + D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate | 172% of the activity with dTMP | Bacillus anthracis | ADP + D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-diphosphate | - |
? | |
ATP + dAMP | 2.7% of the activity with dTMP | Bacillus anthracis | ADP + dADP | - |
? | |
ATP + dCMP | 1% of the activity with dTMP | Bacillus anthracis | ADP + dCDP | - |
? | |
ATP + dTMP | - |
Bacillus anthracis | ADP + dTDP | - |
? | |
ATP + dUMP | 10.7% of the activity with dTMP | Bacillus anthracis | ADP + dUDP | - |
? | |
ATP + IMP | 1% of the activity with dTMP | Bacillus anthracis | ADP + IDP | - |
? | |
ATP + L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate | poor substrate | Bacillus anthracis | ADP + L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-diphosphate | - |
? | |
dATP + dTMP | dATP, 83% of the activity with ATP | Bacillus anthracis | dADP + dTDP | - |
? | |
additional information | no substrate: 3'-azidothymidine 5'-monophosphate | Bacillus anthracis | ? | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
pH 7.6, 37°C | Bacillus anthracis | D-2'-fluoroarabinosyl-5-methyldeoxyuridine |