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Literature summary for 2.7.4.9 extracted from

  • Petit, C.M.; Koretke, K.K.
    Characterization of Streptococcus pneumoniae thymidylate kinase: steady-state kinetics of the forward reaction and isothermal titration calorimetry (2002), Biochem. J., 363, 825-831.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
histidine-tagged enzyme, overexpression in Escherichia coli Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
ADP ADP[beta-S] Streptococcus pneumoniae
dTDP
-
Streptococcus pneumoniae
dTMP
-
Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
dTMP pH 8.5, 25°C Streptococcus pneumoniae
0.235
-
ATP pH 8.5, 25°C Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + dTMP Streptococcus pneumoniae reaction in both the de novo and salvage pathways of dTTP synthesis ADP + dTDP
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate no activity Streptococcus pneumoniae ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate
-
?
ATP + dTMP
-
Streptococcus pneumoniae ADP + dTDP
-
?
ATP + dTMP reaction in both the de novo and salvage pathways of dTTP synthesis Streptococcus pneumoniae ADP + dTDP
-
?
ATP + dUMP 15% of the activity with dTMP Streptococcus pneumoniae ADP + dUDP
-
?
CTP + dTMP 20% of the activity with ATP Streptococcus pneumoniae CDP + dTDP
-
?
dATP + dTMP 80% of the activity with ATP Streptococcus pneumoniae dADP + dTDP
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.9
-
ATP pH 8.5, 25°C Streptococcus pneumoniae
8.9
-
dTMP pH 8.5, 25°C Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Streptococcus pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 40% of maximal activity, pH 9.5: about 55% of maximal activity Streptococcus pneumoniae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.047
-
ADP pH 8.5, 25°C, with ATP as variable substrate Streptococcus pneumoniae
0.16
-
dTMP pH 8.5, 25°C, dTMP as variable substrate Streptococcus pneumoniae
0.415
-
ADP pH 8.5, 25°C, with dTMP as variable substrate Streptococcus pneumoniae