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Literature summary for 2.7.4.9 extracted from
- Mouse ascites sarcoma 180 thymidylate kinase. General properties, kinetic analysis, and inhibition studies (1973), Biochemistry, 12, 2612-2619.
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is very unstable and may be stabilized in the presence of 2-mercaptoethanol with dTMP, dTDP, or ADP but not with ATP, MgATP2- or mercaptoethanol alone | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-aminodeoxythymidine | - |
Mus musculus |  |
| 5'-carboxyldeoxythymidine | - |
Mus musculus | |
| 5'-chlorodeoxythymidine | - |
Mus musculus | |
| 5'-iododeoxythymidine | - |
Mus musculus | |
| ADP | - |
Mus musculus | |
| dTTP | potent feedback inhibitor of dTMP | Mus musculus | |
| TDP | - |
Mus musculus | |
| thymidine | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
MgdATP2- | - |
Mus musculus | |
| 1.5 | - |
ATP | pH 7.5, 37°C | Mus musculus | |
| 1.5 | - |
ATP | ATP in form of MgATP2- | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
sucrose density gradient centrifugation | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| S-180 cell | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.024 | - |
- |
Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 1 week without significant loss of activity | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dTMP | - |
Mus musculus | ADP + dTDP | - |
? | |
| CTP + dTMP | no activity with CTP | Mus musculus | CDP + dTDP | - |
? | |
| dATP + dTMP | - |
Mus musculus | dADP + dTDP | - |
? | |
| dCTP + dTMP | no activity with dCTP | Mus musculus | dCDP + dTDP | - |
? | |
| dGTP + dTMP | no activity with dGTP | Mus musculus | dGDP + dTDP | - |
? | |
| GTP + dTMP | no activity with GTP | Mus musculus | GDP + dTDP | - |
? | |
| UTP + dTMP | no activity with UTP | Mus musculus | UDP + dTDP | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 41 | - |
10 min, inactivation when dTMP concentration is below 0.05 mM | Mus musculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
5'-chlorodeoxythymidine | pH 7.5, 37°C | Mus musculus | |
| 0.027 | - |
dTTP | pH 7.5, 37°C | Mus musculus | |
| 0.03 | - |
dTDP | pH 7.5, 37°C | Mus musculus | |
| 0.03 | - |
5'-carboxyldeoxythymidine | pH 7.5, 37°C | Mus musculus | |
| 0.13 | - |
5'-aminodeoxythymidine | pH 7.5, 37°C | Mus musculus | |
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