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Literature summary for 2.7.4.9 extracted from

  • Tamiya, N.; Yusa, T.; Yamaguchi, Y.; Tsukifuji, R.; Kuroiwa, N.; Moriyama, Y.; Fujimura, S.
    Co-purification of thymidylate kinase and cytosolic thymidine kinase from human term placenta by affinity chromatography (1989), Biochim. Biophys. Acta, 995, 28-35.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP 0.5 mM, 33% inhibition Homo sapiens
dADP 0.5 mM, 34% inhibition Homo sapiens
dGDP 0.5 mM, 23% inhibition Homo sapiens
dTDP 0.5 mM, 91% inhibition Homo sapiens
dUDP 0.5 mM, 42% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0049
-
dTMP pH 7.4, 37°C Homo sapiens
0.62
-
ATP pH 7.4, 37°C Homo sapiens
0.62
-
ATP ATP in form of MgATP2- Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
2 * 24000, SDS-PAGE Homo sapiens
50000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
placenta term placenta Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.833
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dTMP
-
Homo sapiens ADP + dTDP
-
?
CTP + dTMP 56% of the activity with ATP Homo sapiens CDP + dTDP
-
?
dATP + dTMP 99% of the activity with ATP Homo sapiens dADP + dTDP
-
?
dCTP + dTMP 45% of the activity with ATP Homo sapiens dCDP + dTDP
-
?
dGTP + dTMP 59% of the activity with ATP Homo sapiens dGDP + dTDP
-
?
GTP + dTMP 60% of the activity with ATP Homo sapiens GDP + dTDP
-
?
UTP + dTMP 60% of the activity with ATP Homo sapiens UDP + dTDP
-
?

Subunits

Subunits Comment Organism
dimer 2 * 24000, SDS-PAGE Homo sapiens