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Literature summary for 2.7.4.8 extracted from

  • Khan, N.; Shah, P.P.; Ban, D.; Trigo-Mourino, P.; Carneiro, M.G.; DeLeeuw, L.; Dean, W.L.; Trent, J.O.; Beverly, L.J.; Konrad, M.; Lee, D.; Sabo, T.M.
    Solution structure and functional investigation of human guanylate kinase reveals allosteric networking and a crucial role for the enzyme in cancer (2019), J. Biol. Chem., 294, 11920-11933 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli C41(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
G3A decrease in Tm-value by 1.4°C as compared to wild-type enzyme. Decrease in catalytic efficiency (kcat/Km) by 14% Homo sapiens
R116Q decrease in Tm-value by 1.8°C as compared to wild-type enzyme. Decrease in catalytic efficiency (kcat/Km) by 18% Homo sapiens
R96H decrease in Tm-value by 4°C as compared to wild-type enzyme. Increase in catalytic efficiency (kcat/Km) by 22% Homo sapiens
S121F decrease in Tm-value by 3.6°C as compared to wild-type enzyme. Decrease in catalytic efficiency (kcat/Km) by 25% Homo sapiens
S186Y decrease in Tm-value by 2.8°C as compared to wild-type enzyme. Decrease in catalytic efficiency (kcat/Km) by 7% Homo sapiens
S2L decrease in Tm-value by 1.9°C as compared to wild-type enzyme. Decrease in catalytic efficiency (kcat/Km) by 11% Homo sapiens
V91M decrease in Tm-value by 3°C as compared to wild-type enzyme. Decrease in catalytic efficiency (kcat/Km) by 14% Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0207
-
 GMP pH 7.5, 25°C, wild-type enzyme Homo sapiens
0.0296
-
 GMP pH 7.5, 25°C, mutant enzyme R96G Homo sapiens
0.0315
-
 GMP pH 7.5, 25°C, mutant enzyme S2L Homo sapiens
0.0368
-
 GMP pH 7.5, 25°C, mutant enzyme S186Y Homo sapiens
0.0486
-
 GMP pH 7.5, 25°C, mutant enzyme S121F Homo sapiens
0.0543
-
 GMP pH 7.5, 25°C, mutant enzyme R116Q Homo sapiens
0.0547
-
 GMP pH 7.5, 25°C, mutant enzyme V91M Homo sapiens
0.0783
-
 GMP pH 7.5, 25°C, mutant enzyme G3A Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + GMP Homo sapiens
-
 ADP + GDP
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16774
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GMP
-
 Homo sapiens ADP + GDP
-
 ?

Synonyms

Synonyms Comment Organism
GMPK
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42.4
-
 Tm value, mutant enzyme R96H Homo sapiens
43.8
-
 Tm value, mutant enzyme S121F Homo sapiens
44.4
-
 Tm value, mutant enzyme V91M Homo sapiens
44.6
-
 Tm value, mutant enzyme S186Y Homo sapiens
45.5
-
 Tm value, mutant enzyme S2L Homo sapiens
45.6
-
 Tm value, mutant enzyme R116Q Homo sapiens
46
-
 Tm value, mutant enzyme G3A Homo sapiens
47.4
-
 Tm value, wild-type enzyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
58.5
-
 GMP pH 7.5, 25°C, wild-type enzyme Homo sapiens
77.7
-
 GMP pH 7.5, 25°C, mutant enzyme S2L Homo sapiens
96.5
-
 GMP pH 7.5, 25°C, mutant enzyme S186Y Homo sapiens
100.6
-
 GMP pH 7.5, 25°C, mutant enzyme R96G Homo sapiens
101.4
-
 GMP pH 7.5, 25°C, mutant enzyme S121F Homo sapiens
127
-
 GMP pH 7.5, 25°C, mutant enzyme R116Q Homo sapiens
132.2
-
 GMP pH 7.5, 25°C, mutant enzyme V91M Homo sapiens
184.4
-
 GMP pH 7.5, 25°C, mutant enzyme G3A Homo sapiens

General Information

General Information Comment Organism
metabolism critical role in metabolic activation of antiviral and antineoplastic nucleoside-analog prodrugs Homo sapiens
metabolism the only known enzyme responsible for cellular GDP production, making it essential for cellular viability and proliferation Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2100
-
 GMP pH 7.5, 25°C, mutant enzyme S121F Homo sapiens
2300
-
 GMP pH 7.5, 25°C, mutant enzyme R116Q Homo sapiens
2400
-
 GMP pH 7.5, 25°C, mutant enzyme G3A Homo sapiens
2400
-
 GMP pH 7.5, 25°C, mutant enzyme V91M Homo sapiens
2500
-
 GMP pH 7.5, 25°C, mutant enzyme S2L Homo sapiens
2600
-
 GMP pH 7.5, 25°C, mutant enzyme S186Y Homo sapiens
2800
-
 GMP pH 7.5, 25°C, wild-type enzyme Homo sapiens
3400
-
 GMP pH 7.5, 25°C, mutant enzyme R96G Homo sapiens