BRENDA - Enzyme Database
show all sequences of 2.7.4.7

Biosynthesis of hydroxymethylpyrimidine pyrophosphate in Saccharomyces cerevisiae

Kawasaki, Y.; Onozuka, M.; Mizote, T.; Nosaka, K.; Curr. Genet. 47, 156-162 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
Saccharomyces cerevisiae
enzyme is required for thiamin biosynthesis
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
?
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
Saccharomyces cerevisiae YPH500
enzyme is required for thiamin biosynthesis
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
enzyme is required for thiamin biosynthesis
661537
Saccharomyces cerevisiae
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
-
?
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
the Thi20p protein has a greater ability than the Thi21p protein to catalyze the reaction
661537
Saccharomyces cerevisiae
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
-
?
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
enzyme is required for thiamin biosynthesis
661537
Saccharomyces cerevisiae YPH500
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
-
?
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
the Thi20p protein has a greater ability than the Thi21p protein to catalyze the reaction
661537
Saccharomyces cerevisiae YPH500
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
Saccharomyces cerevisiae
enzyme is required for thiamin biosynthesis
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
?
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
Saccharomyces cerevisiae YPH500
enzyme is required for thiamin biosynthesis
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
enzyme is required for thiamin biosynthesis
661537
Saccharomyces cerevisiae
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
-
?
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
the Thi20p protein has a greater ability than the Thi21p protein to catalyze the reaction
661537
Saccharomyces cerevisiae
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
-
?
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
enzyme is required for thiamin biosynthesis
661537
Saccharomyces cerevisiae YPH500
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
-
?
ATP + 4-amino-2-methyl-phosphomethylpyrimidine
the Thi20p protein has a greater ability than the Thi21p protein to catalyze the reaction
661537
Saccharomyces cerevisiae YPH500
ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine
-
-
-
?
Other publictions for EC 2.7.4.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722048
Nodwell
Rugulactone and its analogues ...
Staphylococcus aureus
ChemBioChem
13
1439-1446
2012
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3
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1
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3
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3
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-
718477
French
Structure of trifunctional THI ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. D
67
784-791
2011
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1
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1
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1
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686824
Onozuka
Involvement of thiaminase II e ...
Saccharomyces cerevisiae
FEMS Yeast Res.
8
266-275
2008
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1
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1
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1
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1
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1
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1
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1
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1
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1
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2
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1
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1
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-
661316
Haas
Thi20, a remarkable enzyme fro ...
Saccharomyces cerevisiae
Bioorg. Chem.
33
338-344
2005
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
661537
Kawasaki
Biosynthesis of hydroxymethylp ...
Saccharomyces cerevisiae
Curr. Genet.
47
156-162
2005
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1
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2
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1
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1
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4
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1
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2
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1
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4
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645187
Cheng
Crystal structure of 4-amino-5 ...
Salmonella enterica subsp. enterica serovar Typhimurium
Structure
10
225-235
2002
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1
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1
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1
1
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2
1
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1
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1
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2
1
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639726
Kim
A Brassica cDNA clone encoding ...
Brassica napus
Plant Mol. Biol.
37
955-966
1998
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1
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1
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1
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10
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2
1
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1
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1
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1
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2
1
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641660
Reddick
-
Overexpression, purification a ...
Escherichia coli
Tetrahedron
54
15983-15991
1998
-
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1
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3
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2
2
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1
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1
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3
1
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3
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1
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3
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2
2
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1
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3
1
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3
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641657
Lewin
-
The biosynthesis of thiamine. ...
Saccharomyces cerevisiae
J. Biol. Chem.
236
2768-2771
1961
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1
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1
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