Literature summary for 2.7.4.6 extracted from

Qian, L.; Liu, X.
Purification, characterization and structure of nucleoside diphosphate kinase from Drosophila melanogaster (2014), Protein Expr. Purif., 103, 48-55.

Cloned(Commentary)

Cloned (Comment)	Organism
gene awd, DNA and amino acid sequence determinatioon and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha	Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged wild-type and selenomethionine-labeled enzymes, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 50 mM Tris, 500 mM NaCl, pH 8.0, with 0.001 ml of precipitatant solution, followed by equilibration aganst 0.1 ml reservoir solution containing 60% v/v Tacsimate, pH 7.0, 20°C, X-ray diffraction structure determination and analysis at 1.39-1.94 A resolution	Drosophila melanogaster

Crystallization (Comment)

Organism

purified recombinant His-tagged wild-type and selenomethionine-labeled enzymes, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 50 mM Tris, 500 mM NaCl, pH 8.0, with 0.001 ml of precipitatant solution, followed by equilibration aganst 0.1 ml reservoir solution containing 60% v/v Tacsimate, pH 7.0, 20°C, X-ray diffraction structure determination and analysis at 1.39-1.94 A resolution

Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	recombinant wild-type enzyme, gel filtration	Drosophila melanogaster
63500	-	recombinant wild-type enzyme, analytical ultracentrifugation	Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + ADP	Drosophila melanogaster	-	ADP + ATP	-	r
ATP + GDP	Drosophila melanogaster	-	ADP + GTP	-	r

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	P08879	gene awd	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and selenomethionine-labeled enzymes to homogeneity from Escherichia coli by nickel affinity chromatography, cation exchange chromatography, and gel filtration	Drosophila melanogaster

Source Tissue

Source Tissue	Comment	Organism	Textmining
Schneider 2 cell	S2 cell	Drosophila melanogaster	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + ADP	-	Drosophila melanogaster	ADP + ATP	-	r
ATP + GDP	-	Drosophila melanogaster	ADP + GTP	-	r
additional information	binding affinity of NDPs with the recombinant enzyme measured by isothermal titration calorimetry, indicate that the purines nucleotides show higher binding affinity compared with pyrimidines. The recombinant enzyme rNDPK has a definite nuclease activity in vitro, which cleaves supercoiled plasmid DNA, but has no effect on dsDNA and ssDNA. No or poor activity with CDP and UDP	Drosophila melanogaster	?	-	?

Subunits

Subunits	Comment	Organism
More	the asymmetric enzyme unit is made of three molecules, each of which consists of a four-stranded anti-parallel beta-sheets and seven alpha-helices, the simulated nucleotide-binding active site is conserved, structure analysis and modelling, overview	Drosophila melanogaster
trimer	gel filtration and analytical ultracentrifugation	Drosophila melanogaster

Synonyms

Synonyms	Comment	Organism
NDPK	-	Drosophila melanogaster
nucleoside diphosphate kinase	-	Drosophila melanogaster

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Drosophila melanogaster