Cloned (Comment) | Organism |
---|---|
- |
Pyrobaculum aerophilum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method at room temperature | Pyrobaculum aerophilum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66–100 and 156–165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor. A structural analysis of the evolved NDP kinase in conjunction with mutagenesis experiments suggests that the substrate specificity of the enzyme is related to the presence of these two inserts | Pyrobaculum aerophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum aerophilum | Q8ZWY4 | - |
- |
Pyrobaculum aerophilum DSM 7523 | Q8ZWY4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum aerophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + ADP | at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C | Pyrobaculum aerophilum | dADP + ATP | - |
? | |
dATP + ADP | at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C | Pyrobaculum aerophilum DSM 7523 | dADP + ATP | - |
? | |
dCTP + ADP | at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C | Pyrobaculum aerophilum | dCDP + ATP | - |
? | |
dCTP + ADP | at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C | Pyrobaculum aerophilum DSM 7523 | dCDP + ATP | - |
? | |
dGTP + ADP | the wild-type enzyme is not active when using dGTP as the phosphate donor. The Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66–100 and 156–165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor | Pyrobaculum aerophilum | dGDP + ATP | - |
? | |
dGTP + ADP | the wild-type enzyme is not active when using dGTP as the phosphate donor. The Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66–100 and 156–165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor | Pyrobaculum aerophilum DSM 7523 | dGDP + ATP | - |
? | |
dTTP + ADP | at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C | Pyrobaculum aerophilum | dTDP + ATP | - |
? | |
dTTP + ADP | at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C | Pyrobaculum aerophilum DSM 7523 | dTDP + ATP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NDP kinase | - |
Pyrobaculum aerophilum |