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Literature summary for 2.7.4.6 extracted from

  • Pédelacq, J.D.; Waldo, G.S.; Cabantous, S.; Liong, E.C.; Terwilliger, T.C.
    Structural and functional features of an NDP kinase from the hyperthermophile crenarchaeon Pyrobaculum aerophilum (2005), Protein Sci., 14, 2562-2573.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Pyrobaculum aerophilum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method at room temperature Pyrobaculum aerophilum

Protein Variants

Protein Variants Comment Organism
additional information the Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66–100 and 156–165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor. A structural analysis of the evolved NDP kinase in conjunction with mutagenesis experiments suggests that the substrate specificity of the enzyme is related to the presence of these two inserts Pyrobaculum aerophilum

Organism

Organism UniProt Comment Textmining
Pyrobaculum aerophilum Q8ZWY4
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-
Pyrobaculum aerophilum DSM 7523 Q8ZWY4
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-

Purification (Commentary)

Purification (Comment) Organism
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Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dATP + ADP at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C Pyrobaculum aerophilum dADP + ATP
-
?
dATP + ADP at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C Pyrobaculum aerophilum DSM 7523 dADP + ATP
-
?
dCTP + ADP at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C Pyrobaculum aerophilum dCDP + ATP
-
?
dCTP + ADP at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C Pyrobaculum aerophilum DSM 7523 dCDP + ATP
-
?
dGTP + ADP the wild-type enzyme is not active when using dGTP as the phosphate donor. The Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66–100 and 156–165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor Pyrobaculum aerophilum dGDP + ATP
-
?
dGTP + ADP the wild-type enzyme is not active when using dGTP as the phosphate donor. The Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66–100 and 156–165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor Pyrobaculum aerophilum DSM 7523 dGDP + ATP
-
?
dTTP + ADP at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C Pyrobaculum aerophilum dTDP + ATP
-
?
dTTP + ADP at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C Pyrobaculum aerophilum DSM 7523 dTDP + ATP
-
?

Synonyms

Synonyms Comment Organism
NDP kinase
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Pyrobaculum aerophilum