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Literature summary for 2.7.4.6 extracted from
- Facilitated folding and subunit assembly in Escherichia coli and in vitro of nucleoside diphosphate kinase from extremely halophilic archaeon conferred by amino-terminal extension containing hexa-His-tag (2004), FEBS Lett., 570, 87-92.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| fusion of extra sequence containing hexa-His-tag at amino-terminus of the enzyme facilitates folding and activation in Escherichia coli | Halobacterium salinarum |
General Stability
| General Stability | Organism |
|---|---|
| amino-terminal extension enhances dimer to hexamer assembly and stabilizes the structure in low salt | Halobacterium salinarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halobacterium salinarum | P61136 | - |
- |
| Halobacterium salinarum JCM 11081 | P61136 | - |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| refolding of the His-tagged enzyme after denaturation is much more efficient than refolding of the untagged enzyme | Halobacterium salinarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 340 | - |
pH 8.0, temperature not specified in the publication | Halobacterium salinarum |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | folded His-tagged enzyme isolated from Escherichia coli forms a hexamer in both 0.2 M and 3.8 M NaCl at 30 °C, while the native enzyme purified from Halobacterium salinarum dissociates to dimer in 0.2 M NaCl | Halobacterium salinarum |
| hexamer | folded His-tagged enzyme isolated from Escherichia coli forms a hexamer in both 0.2 M and 3.8 M NaCl at 30 °C, while the native enzyme purified from Halobacterium salinarum dissociates to dimer in 0.2 M NaCl | Halobacterium salinarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HsNDK | - |
Halobacterium salinarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Halobacterium salinarum |
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