Protein Variants | Comment | Organism |
---|---|---|
N111L | a HisN111L mutant with an N-terminal extension sequence containing hexa-His tag has enhanced salt requirements for enzymatic activity and refolding even though the secondary structure of the HisN111L mutant is confirmed to be similar to the control | Halobacterium salinarum |
General Stability | Organism |
---|---|
the wild-type enzyme requires NaCl at higher than 2 M NaCl for refolding. Increasing the salt concentration gradually facilitates the recovery of enzyme activity, and the recovery at 3 M NaCl is maximal. The recovery at 4 M NaCl decreases slightly. No refolding of the HisN111L mutant is detected below 1 M NaCl and slow refolding is observed at 2 M NaCl, significant recovery occurs at higher than 3 M NaCl. The refolding efficiency at 4 M NaCl is as same as that of 3 M NaCl, differently from the wild-type enzyme | Halobacterium salinarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require NaCl for stability or enzymatic activity below 30°C | Halobacterium salinarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium salinarum | P61136 | - |
- |
Halobacterium salinarum JCM 11081 | P61136 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Halobacterium salinarum |
Subunits | Comment | Organism |
---|---|---|
homohexamer | assembled as a trimer of basic dimeric units | Halobacterium salinarum |
Synonyms | Comment | Organism |
---|---|---|
nucleoside diphosphate kinase | - |
Halobacterium salinarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 53 | the melting of the HisN111L mutant shows a transition starting at 45.2°C and ending at 52.6°C in 0.2 M NaCl | Halobacterium salinarum |
47 | 52 | the melting of the wild-type enzyme shows a transition starting at 46.7°C and ending at 51.9°C in 0.2 M NaCl | Halobacterium salinarum |