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Literature summary for 2.7.4.6 extracted from

  • Arai, S.; Yonezawa, Y.; Okazaki, N.; Matsumoto, F.; Tamada, T.; Tokunaga, H.; Ishibashi, M.; Blaber, M.; Tokunaga, M.; Kuroki, R.
    A structural mechanism for dimeric to tetrameric oligomer conversion in Halomonas sp. nucleoside diphosphate kinase (2012), Protein Sci., 21, 498-510.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star(DE3) cells Halomonas sp. #593

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, crystal form 1 is a cubic shaped crystal grown from 0.2M ammonium acetate, 0.1M sodium citrate tribasic dehydrate at pH 5.6, and 30%(w/v) polyethylene glycol (PEG) 4000, while crystal form 2 is a rod-shaped crystal grown from 0.19M sodium acetate, 0.09M Tris HCl at pH 8.5, and 28%(w/v) PEG 4000 Halomonas sp. #593

Protein Variants

Protein Variants Comment Organism
E134A the mutant forms a tetramer and shows 119% specific activity compared to the wild type enzyme Halomonas sp. #593

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15137
-
2 * 15137, calculated from amino acid sequence Halomonas sp. #593
32100
-
gel filtration and multiangle laser light scattering Halomonas sp. #593

Organism

Organism UniProt Comment Textmining
Halomonas sp. #593 Q83WH5
-
-

Purification (Commentary)

Purification (Comment) Organism
ATP-agarose column chromatography and Hi-Trap Q resin column chromatography Halomonas sp. #593

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + TDP
-
Halomonas sp. #593 ADP + TTP
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 15137, calculated from amino acid sequence Halomonas sp. #593

Synonyms

Synonyms Comment Organism
NDK
-
Halomonas sp. #593
nucleoside diphosphate kinase
-
Halomonas sp. #593