Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star(DE3) cells | Halomonas sp. #593 |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, crystal form 1 is a cubic shaped crystal grown from 0.2M ammonium acetate, 0.1M sodium citrate tribasic dehydrate at pH 5.6, and 30%(w/v) polyethylene glycol (PEG) 4000, while crystal form 2 is a rod-shaped crystal grown from 0.19M sodium acetate, 0.09M Tris HCl at pH 8.5, and 28%(w/v) PEG 4000 | Halomonas sp. #593 |
Protein Variants | Comment | Organism |
---|---|---|
E134A | the mutant forms a tetramer and shows 119% specific activity compared to the wild type enzyme | Halomonas sp. #593 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15137 | - |
2 * 15137, calculated from amino acid sequence | Halomonas sp. #593 |
32100 | - |
gel filtration and multiangle laser light scattering | Halomonas sp. #593 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halomonas sp. #593 | Q83WH5 | - |
- |
Purification (Comment) | Organism |
---|---|
ATP-agarose column chromatography and Hi-Trap Q resin column chromatography | Halomonas sp. #593 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + TDP | - |
Halomonas sp. #593 | ADP + TTP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 15137, calculated from amino acid sequence | Halomonas sp. #593 |
Synonyms | Comment | Organism |
---|---|---|
NDK | - |
Halomonas sp. #593 |
nucleoside diphosphate kinase | - |
Halomonas sp. #593 |