Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
dTDP | - |
Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the presence of Mg2+ enhances enthalpy changes by stabilizing water molecule hydrogen bonding network, resulting in favorable enthalpy changes, the absence of Mg2+ induces a noticeable loss in binding enthalpies and positive favorable entropic changes in GDP binding, indicating a more unstable enzyme-ligand complex formation and lesser complex tightening | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
SDS-PAGE | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NDP | Plasmodium falciparum | - |
ADP + NTP | - |
? | |
ATP + NDP | Plasmodium falciparum FCR-3 | - |
ADP + NTP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Plasmodium falciparum FCR-3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity resin column chromatography and Sephacryl S300 gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dTDP | - |
Plasmodium falciparum | ADP + dTTP | - |
? | |
ATP + dTDP | - |
Plasmodium falciparum FCR-3 | ADP + dTTP | - |
? | |
ATP + GDP | - |
Plasmodium falciparum | ADP + GTP | - |
? | |
ATP + GDP | - |
Plasmodium falciparum FCR-3 | ADP + GTP | - |
? | |
ATP + NDP | - |
Plasmodium falciparum | ADP + NTP | - |
? | |
ATP + NDP | - |
Plasmodium falciparum FCR-3 | ADP + NTP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NDK | - |
Plasmodium falciparum |
nucleoside diphosphate kinase | - |
Plasmodium falciparum |
nucleoside diphosphate phosphotransferase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 42 | - |
Plasmodium falciparum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | enzyme activity is decreased by 70% at 50°C and completely lost after preincubation at 60°C, irreversible loss of activity is detected after treating the samples at temperatures above 70°C | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.2 | - |
Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Plasmodium falciparum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | the activity markedly drops as pH exceeds 7.8, about 25% relative activity at pH8.0-9.0 | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Plasmodium falciparum |