Literature summary for 2.7.4.6 extracted from

Moynie, L.; Giraud, M.; Georgescauld, F.; Lascu, I.; Dautant, A.
The structure of the Escherichia coli nucleoside diphosphate kinase reveals a new quaternary architecture for this enzyme family (2007), Proteins, 67, 755-765.

Cloned(Commentary)

Cloned (Comment)	Organism
gene ndk, overexpressionin strain BL21(DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, sitting drop vapor diffusion method, mixing 200 nL of protein solution, containing 15 mg/mL protein, with 200 nL of reservoir solution containing 0.2 M ammonium sulphate, 0.1 M sodium acetate/acetic acid buffer, pH 4.6, with either 30% PEG 2000 monomethyl ether or 25% PEG 4000, 20°C, a few hours, X-ray diffraction structure determination and analysis at 1.62 A resolution	Escherichia coli

Crystallization (Comment)

Organism

purified recombinant enzyme, sitting drop vapor diffusion method, mixing 200 nL of protein solution, containing 15 mg/mL protein, with 200 nL of reservoir solution containing 0.2 M ammonium sulphate, 0.1 M sodium acetate/acetic acid buffer, pH 4.6, with either 30% PEG 2000 monomethyl ether or 25% PEG 4000, 20°C, a few hours, X-ray diffraction structure determination and analysis at 1.62 A resolution

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	gene ndk	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from strain BL21(DE3) by anion exchange chromatography, ammonium sulfate fractionation, dialysis, and gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	NDPK catalyzes the transfer of gamma-phosphate from nucleoside triphosphates to nucleoside diphosphates	Escherichia coli	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	the enzyme shows a tetrameric quaternary structure, the relative orientation of interacting dimers facing either the convex or the concave side of their central sheet, overview	Escherichia coli

Synonyms

Synonyms	Comment	Organism
NDPK	-	Escherichia coli
nucleoside diphosphate kinase	-	Escherichia coli