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Literature summary for 2.7.4.6 extracted from

  • Kolli, B.K.; Kostal, J.; Zaborina, O.; Chakrabarty, A.M.; Chang, K.P.
    Leishmania-released nucleoside diphosphate kinase prevents ATP-mediated cytolysis of macrophages (2008), Mol. Biochem. Parasitol., 158, 163-175.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.6

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged enzyme containing a thrombin cleavage site Leishmania amazonensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Leishmania amazonensis 5829
-
extracellular enzyme secretion Leishmania amazonensis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Leishmania amazonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 4 * 17000, about, SDS-PAGE Leishmania amazonensis
65000 68000
-
 Leishmania amazonensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Leishmania amazonensis NdK is a stable enzyme capable of decreasing extracellular ATP levels ?
-
 ?
additional information Leishmania amazonensis LV78 NdK is a stable enzyme capable of decreasing extracellular ATP levels ?
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania amazonensis
-
 clone 12-1, gene ndk
-
Leishmania amazonensis LV78
-
 clone 12-1, gene ndk
-

Purification (Commentary)

Purification (Comment) Organism
cytosolic and secreted enzymes 76fold and 238fold, respectively, recombinant His6-tagged enzyme by nickel affinity chromatography Leishmania amazonensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
207
-
 purified extracellular enzyme Leishmania amazonensis
6427
-
 purified cytosolic enzyme Leishmania amazonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CDP
-
 Leishmania amazonensis ADP + CTP
-
 r
ATP + CDP
-
 Leishmania amazonensis LV78 ADP + CTP
-
 r
ATP + GDP
-
 Leishmania amazonensis ADP + GTP
-
 r
ATP + GDP
-
 Leishmania amazonensis LV78 ADP + GTP
-
 r
ATP + UDP
-
 Leishmania amazonensis ADP + UTP
-
 r
ATP + UDP
-
 Leishmania amazonensis LV78 ADP + UTP
-
 r
additional information NdK is a stable enzyme capable of decreasing extracellular ATP levels Leishmania amazonensis ?
-
 ?
additional information both recombinant and native Leishmania NdK utilized ATP to produce expected nucleoside triphosphates in the presence of nucleoside diphosphates in excess Leishmania amazonensis ?
-
 ?
additional information NdK is a stable enzyme capable of decreasing extracellular ATP levels Leishmania amazonensis LV78 ?
-
 ?
additional information both recombinant and native Leishmania NdK utilized ATP to produce expected nucleoside triphosphates in the presence of nucleoside diphosphates in excess Leishmania amazonensis LV78 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 17000, about, SDS-PAGE Leishmania amazonensis

Synonyms

Synonyms Comment Organism
NDK
-
 Leishmania amazonensis
nucleoside diphosphate kinase
-
 Leishmania amazonensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Leishmania amazonensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Leishmania amazonensis